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Enzyme Substrates

Enzyme Substrates

Enzyme substrates are molecules upon which enzymes act to catalyze chemical reactions. The substrate binds to the active site of the enzyme, where it undergoes a specific transformation to form the product of the reaction. Enzyme substrates are essential tools in research for studying enzyme kinetics, specificity, and mechanism of action. They are also used in diagnostic assays and biochemical studies. At CymitQuimica, we provide a diverse selection of high-quality enzyme substrates to support your research in enzymology and biochemistry.

Subcategories of "Enzyme Substrates"

Found 1526 products of "Enzyme Substrates"

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  • L-Lysyl-L-alanine 7-amido-4-methylcoumarin dihydrochloride

    CAS:
    L-Lysyl-L-alanine 7-amido-4-methylcoumarin dihydrochloride is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.
    Purity:Min. 98.0 Area-%
    Color and Shape:Powder

    Ref: 3D-EL172292

    1g
    1,551.00€
    5g
    5,710.00€
    250mg
    574.00€
    500mg
    946.00€
    2500mg
    3,284.00€
  • Luc-Enterobacteria Na


    Luc-Enterobacteria Na is a bioluminescent substrate for bacterial luciferase used to detect Enterobacteriaceae. The enzyme luciferase oxidizes luciferin with FMNH₂ and O₂ via luxAB genes, emitting blue light.
    Formula:C17H17NaN2O8S2
    Purity:Min. 94 Area-%
    Molecular weight:464.44 g/mol

    Ref: 3D-L-8278

    1g
    8,489.00€
    50mg
    1,063.00€
    100mg
    1,778.00€
    250mg
    3,546.00€
    500mg
    5,751.00€
  • 5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside

    CAS:
    5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in glycolipid metabolism studies.
    Formula:C14H15BrClNO6
    Color and Shape:White to off-white solid.
    Molecular weight:408.63 g/mol

    Ref: 3D-EB03261

    1g
    641.00€
    5g
    1,988.00€
    10g
    3,227.00€
    25g
    6,721.00€
    2500mg
    1,193.00€
  • 7-((4'-L-Alaninamido)-rac-3'-hydroxybutyloxy) coumarin hydrochloride


    This Clips-O substrate 7-((4'-L-Alaninamido)-rac-3'-hydroxybutyloxy) coumarin allows the detection of single and dual enzymatic activity (L-alanine aminopetidase and oxidation of 4-amino-3-hydroxybutyloxy spacer either enzymatically or by exposure to sodium periodate) with a single substrate resulting in a fluorescent signal. The main virtues of Clips-O substrates are an increased stability towards non-enzymatic hydrolysis that results in a reduced background and high sensitivity.
    Formula:C16H21ClN2O5
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:356.8 g/mol

    Ref: 3D-EA183107

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  • 6-Chloro-3-indoxyl-beta-D-galactopyranoside

    CAS:
    6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.
    Formula:C14H16ClNO6
    Purity:Min. 98.0 Area-%
    Molecular weight:329.74 g/mol

    Ref: 3D-C-5000

    1g
    542.00€
    2g
    821.00€
    5g
    1,451.00€
    10g
    1,934.00€
    25g
    3,531.00€
  • 4-Methylumbelliferyl heptanoate

    CAS:
    Lipase targeting MOSCERDAM® substrate
    Formula:C17H20O4
    Purity:Min. 95%
    Color and Shape:White Powder
    Molecular weight:288.34 g/mol

    Ref: 3D-EM67746

    1g
    348.00€
    2g
    488.00€
    5g
    798.00€
    10g
    1,285.00€
    25g
    2,343.00€
  • 5-Nitro-3-indoxyl-β-D-galactopyranoside


    For the detection of beta-galactosidase activity
    Formula:C14H16N2O8
    Molecular weight:340.29 g/mol

    Ref: 3D-N-2595

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  • 6-Nitro-3-indoxyl-β-D-galactopyranoside


    For the detection of beta-galactosidase activity
    Formula:C14H16N2O8
    Molecular weight:340.29 g/mol

    Ref: 3D-N-2596

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  • AquaSpark® 510 Calibration Probe, Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191

    CAS:

    AquaSpark® Calibration Probe is a water-soluble dioxetane-based chemiluminescent probe. Deprotonation of the free phenol group of the calibration probe under mild basic conditions results in an emissive decomposition process. No addition of enhancer or sensitizing agent is required to produce the bright green light emission in aqueous solutions.
    AquaSpark® Calibration Probe is used for:

    Demonstration experiment susing only the probe and an aqueous solution or water;
    Evaluation of the AquaSpark® maximum light emission;
    Functional tests of luminometers, plate readers and cameras;
    Comparison with other probes bearing an enzyme labile group in order to build concentration/response curves;
    Validation and comparison between different AquaSpark® probes.

    Formula:C21H23ClO6
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:406.12 g/mol

    Ref: 3D-EA175398

    1mg
    264.00€
    2mg
    375.00€
    5mg
    607.00€
    250µg
    153.00€
    500µg
    182.00€
  • Chitin azure

    CAS:

    Chitin azure is a chromogenic substrate for chitinases. It is one of the few specific substrates for this enzyme that produces chito-oligosaccharides from chitin.

    Color and Shape:Powder

    Ref: 3D-EC71613

    1g
    1,018.00€
    250mg
    412.00€
    500mg
    651.00€
  • Resorufin b-D-xylobioside


    Resorufin b-D-xylobioside is a fluorogenic substrate for xylanases useful for xylanase activity assays.
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:477.42 g/mol

    Ref: 3D-ER76453

    1mg
    231.00€
    2mg
    411.00€
    5mg
    819.00€
    10mg
    1,322.00€
  • Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride

    CAS:
    Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride (Gly-Gly-AMC) is a fluorescent compound that can be used as a probe for the transfer of glycine in the brain. Gly-Gly-AMC binds to Langerhans cells and is transferred by an anterograde axonal transport mechanism from the peripheral nervous system to the central nervous system. The binding of Gly-Gly-AMC to Langerhans cells has been shown to activate them, which induces the release of inflammatory mediators into the surrounding tissue. This activity may be due to increased levels of calcium ions, which are known to induce exocytosis in these cells. Glycine is also involved in depression and other mental disorders, as well as muscle and brain activity.
    Formula:C14H16ClN3O4
    Molecular weight:325.75 g/mol

    Ref: 3D-G-6100

    1g
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    5g
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    10g
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    500mg
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    2500mg
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  • Luc-Salmonella


    Luc-Salmonella is a bioluminescent substrate for bacterial luciferase use to detect Salmonella.

    Purity:Min. 95%
    Color and Shape:Powder

    Ref: 3D-EL175446

    5mg
    258.00€
    10mg
    407.00€
    25mg
    713.00€
    50mg
    1,298.00€
  • Ac-Abu-Tle-Leu-Gln-AMC TFA salt


    A fluorogenic substrate for detection of enzymatic activity of the SARS-CoV-2 Mpro protease. The fluorophore 7-Amino-4-methylcoumarin (AMC) is linked to the tetrapeptide via a peptide bond at the C-terminal of the glutamine residue. Following the hydrolytic activity of the Mpro protease, the fluorogenic AMC group get released from the substrate, generating a fluorescent signal (Ex 380 nm / Em 455 nm).
    Formula:C33H48N6O8(freebase)
    Purity:Min. 95.0 Area-%
    Molecular weight:656.77 g/mol

    Ref: 3D-FA178674

    1mg
    249.00€
    2mg
    371.00€
    5mg
    575.00€
    10mg
    853.00€
    25mg
    1,313.00€
  • 4-Methylumbelliferyl-N,N-diacetyl-beta-D-chitobioside

    CAS:
    4-Methylumbelliferyl-N,N-diacetyl-beta-D-chitobioside is a molecule that has been expressed in the yeast Pichia pastoris. It can be used as a fluorescent probe for chitinase activity and has been shown to be sensitive to tryptophan residues and aromatic residues in proteins. 4-Methylumbelliferyl-N,N-diacetyl-beta-D-chitobioside has also been used as a fluorescent probe for isozymes of glucose phosphate isomerase. The nature of the trisaccharide is unknown, but it appears to be hydrophobic.
    Formula:C26H34N2O13
    Purity:Min. 99.0 Area-%
    Molecular weight:582.57 g/mol

    Ref: 3D-M-5532

    25mg
    367.00€
    50mg
    534.00€
    100mg
    885.00€
    250mg
    1,822.00€
    500mg
    3,097.00€
  • 4-(Bromomethyl)phenyl-β-D-triacetylglucuronic acid methyl ester


    4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester, also known as 4-bromo-4'-(N,N-dimethylamino)biphenyl, is a bioluminescent substrate. It is used for the detection of beta-glucuronidase activity in biological samples and for the detection of bacterial β-glucuronidase activity in culture media. Beta-glucuronidase catalyzes the hydrolysis of glucuronic acid by breaking down a conjugate molecule into two smaller molecules, which can be detected using bioluminescence. 4-(Bromomethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester is a chromogenic substrate that has been shown to be an excellent ligand in biomolecular research because it reacts with many different enzymes. This product is highly pure and

    Formula:C20H23BrO10
    Purity:Min. 95 Area-%
    Molecular weight:503.29 g/mol

    Ref: 3D-B-8898

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  • Luc-coliforms Na

    CAS:
    Luc-coliforms Na is a high quality, chromogenic substrate for the detection of coliforms. It is used widely in the food industry to detect whether coliforms are present in milk, cheese, and other dairy products. Luc-coliforms Na provides a color change from yellow to blue when it reacts with coliforms and has a high pH range of 4.0-8.0. Luc-coliforms Na has a CAS number of 93824-43-4.
    Purity:Min. 95%

    Ref: 3D-EL175439

    5mg
    226.00€
    10mg
    355.00€
    25mg
    660.00€
    50mg
    1,138.00€
  • 4-Methylumbelliferyl phosphate, free acid

    CAS:

    A sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. It is used for the detection of C. perfringens because acid phosphatase is a highly specific indicator for C. perfringens.

    Formula:C10H9O6P
    Molecular weight:256.15 g/mol

    Ref: 3D-M-5801

    10g
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    25g
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    50g
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    100g
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    250g
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  • 1-Naphthyl β-D-mannopyranoside

    CAS:
    1-Naphthyl β-D-mannopyranoside is a chromogenic (also fluorogenic) substrate. After cleavage, 1-naphthol is released and is used to quantify beta-mannosidase activity.
    Formula:C16H18O6
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:306.31 g/mol

    Ref: 3D-EN06884

    100mg
    268.00€
    250mg
    475.00€
    500mg
    713.00€
  • AquaSpark® caprylate, 10 mM in DMSO, Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191

    CAS:

    Chemiluminescent substrate for C8-esterase and lipase

    Formula:C36H43ClO8
    Purity:Min. 95%
    Color and Shape:Clear Liquid
    Molecular weight:639.18 g/mol

    Ref: 3D-EA175392

    100µl
    341.00€