Enzyme Substrates

Chemiluminescence substrate for AP detection

2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid, also known as p-nitrophenyl N-acetylneuraminic acid, is a chromogenic substrate specifically designed for the detection and quantification of neuraminidase (sialidase) activity. Upon cleavage by neuraminidase, it releases a yellow-colored product, 4-nitrophenol, which can be easily monitored spectrophotometrically at 405 nm. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of neuraminidase activity in biological samples such as viruses, bacteria, and cell lysates. It is particularly useful for studying the enzymatic properties of neuraminidases from different sources and for the development of novel antiviral and antibacterial agents targeting neuraminidase activity.

Luc-S.aureus K is an enzyme conjugate containing luciferase and a specific antibody against Staphylococcus aureus. It is used for detection of S.aureus in laboratory cultures, food, and environmental samples. The light produced by this product is proportional to the amount of bacteria present in the sample. This product can be used as a chemiluminescent substrate for detecting alkaline phosphatase activity or as a fluoro-luminescent substrate for detecting beta-galactosidase activity. Luc-S.aureus K has high purity and high quality, with 100% specific binding affinity for S.aureus and no cross reactivity with other bacteria or host cells, making it suitable for use in diagnostic applications.

Water soluble substrate for firefly luciferase. Used for imaging the expression of the LUC marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Extensively utilized in reporter gene assays, immunoassays and hygiene monitoring.

Chromogenic beta-Glucosidase substrate yielding a yellow solution upon cleavage.

Water soluble substrate for firefly luciferase. Used for imaging the expression of the LUC marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Extensively utilized in reporter gene assays, immunoassays and hygiene monitoring. Endotoxin free product.

Aldol® 455 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucosidase activity. The colorless enzyme substrate is cleaved by beta-glucosidase producing bacteria grown in liquid or solid media, yielding a yellow color and green to orange fluorescence. Aldol® 455 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.

Please enquire for more information about BCM® Staphylococcus aureus Plating medium including the price, delivery time and more detailed product information at the technical inquiry form on this page

2-Naphthyl-lpha-D-glucopyranoside is a substrate for α-glucosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.

4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-6-sulfate potassium salt is a fluorogenic substrate for N-acetylgalactosamine-6-sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-6-sulfate potassium saltas a substrate for measuring the N-acetylgalactosamine-6-sulfatase activity is used for diagnosis of Mucopolysaccharidosis IVA (MPS IVA) (a rare lysosomal storage disorder) and Morquio A syndrome screening.