Enzyme Substrates

<p>Fast Blue BB is commonly used for assaying beta-glycosidase activity and the chromogenic reaction between Fast Blue BB and beta-naphthol results in a reddish colored product and is at the basis of its application to different enzymatic activity assays. Produces a fluorescent, blue-green chromophore.</p>

<p>Substrates for endoglucanase and beta-cellobiohydrolase activity in the study of digestive system in Zygentoma as an insect model for high cellulase activity</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>4-Nitrophenyl beta-galactoside (pNP-Gal) is a chromogenic substrate commonly used in enzymatic assays to detect and quantify the presence of beta-galactosidase. It is hydrolyzed by the enzyme to release 4-nitrophenol, which produces a yellow color that can be measured spectrophotometrically. Similarly, 4-Nitrophenyl beta-glucoside (pNP-Glc) can be used to assay beta-glucosidase activity. 4-Nitrophenyl beta-globotrioside (pNP-Glo) is a similar compound that has been designed as a substrate for glycosidases that cleave terminal beta-galactosyl-(1-&gt;3)-N-acetylgalactosamine residues. In these assays, the release of 4-nitrophenol can be measured to determine the activity of the enzyme under study. The use of chromogenic substrates like pNP-Glo allows for the rapid and reliable detection of glycosidase activity in a variety of biological samples.</p>

<p>1,8,15,22-Tetrazacyclooctacosane-2,7,16,21-tetraone is an inhibitor with potent anticancer properties. It induces apoptosis in cancer cells by inhibiting kinases that are critical for cell survival and proliferation. This compound has been shown to be effective against various types of cancer in both Chinese hamster ovary and human cancer cell lines. Additionally, it has been found to increase the excretion of the anti-allergic drug rupatadine in urine. The use of 1,8,15,22-Tetrazacyclooctacosane-2,7,16,21-tetraone as a tumor inhibitor has also been investigated in combination with other kinase inhibitors such as bendamustine analogs. This compound holds great potential for the development of new anticancer therapies.</p>

<p>1-Naphthyl-alpha-D-mannopyranoside is a chromogenic substrate. It is used in diagnostics and as a reagent for enzymatic analysis of alpha-mannosidase activity in culture media, food testing, and environmental testing.</p>

<p>2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside is a chromogenic substrate used for beta-N-acetylglucosaminidase assays. Upon enzymatic hydrolysis, 2-chloro-4-nitrophenol is released, which has a yellowish color. It is used in diagnostic applications such as lysosomal storage disorder diagnosis (for example, Tay-Sachs disease) and glycosidase inhibition studies.</p>

<p>2,4-Dinitrophenyl)-L-lysine hydrochloride monohydrate is a lysine derivative that has been modified with a 2,4-dinitrophenyl group. It could be used as an enzyme substrate to monitor enzymes that recognize and modify lysine residues, such as lysine methyltransferases and demethylases, lysine acylases and lysine oxidases.</p>

<p>Remazol Brilliant Blue R D-xylan is a chromogenic substrate used for the detection and quantification of xylanase enzyme activity. This substrate consists of a xylan polymer backbone covalently linked to the Remazol Brilliant Blue R dye. Upon cleavage by xylanase enzymes, the dye is released, producing a vibrant blue color that can be easily measured spectrophotometrically. This substrate is widely used in various applications, including the study of xylanase-producing microorganisms, optimization of enzyme production, and evaluation of enzyme kinetics.</p>

<p>4-Nitrophenyl 2-azido-2-deoxy-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-azido-2-deoxy-beta-D-galactopyranoside is used in different applications such as the Bacterial enzyme assays (e.g., E. coli detection)</p>

<p>2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-a)pyrazin-3-one hydrochloride (ABI) is a potent and selective inhibitor of the enzyme toll-like receptor. The drug has been shown to decrease the metabolic rate in experimental models. ABI has also been used as an analog for other compounds to study their effects on redox potentials and reactive species. It is unclear how ABI inhibits protein synthesis by polymerase chain reaction (PCR). One possible mechanism is that it may inhibit the binding of primers to DNA. ABI also inhibits the production of chemokines which are small proteins that stimulate inflammatory responses. This drug can be used in autoimmune diseases such as rheumatoid arthritis and multiple sclerosis.</p>

<p>COX inhibitor; nonsteroidal anti-inflammatory drug</p>

<p>6-Benzoyl-2-naphthylphosphate disodium salt is a chromogenic substrate used to detect and quantify alkaline phosphatase activity. After enzymatic hydrolysis, 6-benzoyl-2-naphthol is released, which can be detect or even further react with other reagents (e.g. Fast Red) to increase the intensity of the colorimetric signal. It is used in common applications such as ELISA, immunohistochemistry and Western Blotting.</p>

<p>Methyl 4-nitrophenyl hexylphosphonate is a phosphonate inhibitor of the enzyme serine esterase. It has been shown to be more potent than other irreversible inhibitors such as ethyl 4-nitrophenyl hexylphosphonate and methyl 4-nitrophenoxybutyl phosphate. This compound is an immobilized form of the methyl 4-nitrophenyl hexylphosphonate that is immobilized on an inert surface, such as silica gel, via a covalent bonding process. The immobilization of the molecule ensures that it will not leach from the surface and is stable over time. The molecular weight of this substance is 411.5 Daltons and its molecular formula is C8H15NO4P. This substance has a diameter of 0.8 nm and can be found in crystalline form with a melting point of 143 degrees Celsius at atmospheric pressure. Methyl 4-nitrophenyl</p>

<p>Please enquire for more information about 1,2,4-trihydroxynaphthalene-4-O-b-D-glucopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Please enquire for more information about 1,2,4-trihydroxynaphthalene-2-O-b-D-glucopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid, also known as p-nitrophenyl N-acetylneuraminic acid, is a chromogenic substrate specifically designed for the detection and quantification of neuraminidase (sialidase) activity. Upon cleavage by neuraminidase, it releases a yellow-colored product, 4-nitrophenol, which can be easily monitored spectrophotometrically at 405 nm. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of neuraminidase activity in biological samples such as viruses, bacteria, and cell lysates. It is particularly useful for studying the enzymatic properties of neuraminidases from different sources and for the development of novel antiviral and antibacterial agents targeting neuraminidase activity.</p>

<p>The photorelease of 2-deoxy-2-fluoro-L-fucose from 2-nitrobenzyl-2-deoxy-2-fluoro-L-fucopyranoside was performed under a 365 nm LED illumination. It has been shown in plant cells this liberated 2-deoxy-2-fluoro-L-fucose can be converted by the endogenous metabolic machinery into the corresponding nucleotide-sugar, which then efficiently inhibits Golgi-localized fucosyltransferases.</p>

<p>Please enquire for more information about 4-Nitrophenyl valerate including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Bioluminescent substrate for detection of E. coli.</p>