Enzyme Substrates

The AquaSpark® 510 Nitroreductase/NADH Probe (CL-NTR) is a water-soluble probe for the selective chemiluminescent detection of nitroreductase (NTR) activity. It is a versatile tool for monitoring a number of biological processes.

<p>4-Nitrophenyl alpha-D-maltotetraoside is a chromogenic substrate used for the detection of alpha-glucosidase and alpha-amylase activity. The four glucose units in the molecule are hydrolyzed by the enzyme, releasing 4-nitrophenol and allowing for easy colorimetric measurement of enzymatic activity</p>

<p>Fast Blue BB is commonly used for assaying beta-glycosidase activity and the chromogenic reaction between Fast Blue BB and beta-naphthol results in a reddish colored product and is at the basis of its application to different enzymatic activity assays. Produces a fluorescent, blue-green chromophore.</p>

<p>Fluorogenic substrate targeting phosphatase</p>

<p>1-Naphthyl-alpha-D-mannopyranoside is a chromogenic substrate. It is used in diagnostics and as a reagent for enzymatic analysis of alpha-mannosidase activity in culture media, food testing, and environmental testing.</p>

4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(β-D-galactopyranosyl)-α-D-glucopyranoside is a high-quality chromogenic pNP enzyme substrate specifically designed to enable effective and accurate detection of enzyme activity. By utilizing this advanced substrate, researchers and professionals can perform a range of applications, including activity assays, enzyme inhibition studies, and substrate specificity profiling. This substrate's unique properties result in exceptional sensitivity and throughput, ensuring effective results for a wide variety of enzyme assays.

<p>Substrates for endoglucanase and beta-cellobiohydrolase activity in the study of digestive system in Zygentoma as an insect model for high cellulase activity</p>

<p>2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside is a chromogenic substrate used for beta-N-acetylglucosaminidase assays. Upon enzymatic hydrolysis, 2-chloro-4-nitrophenol is released, which has a yellowish color. It is used in diagnostic applications such as lysosomal storage disorder diagnosis (for example, Tay-Sachs disease) and glycosidase inhibition studies.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>4-Nitrophenyl beta-galactoside (pNP-Gal) is a chromogenic substrate commonly used in enzymatic assays to detect and quantify the presence of beta-galactosidase. It is hydrolyzed by the enzyme to release 4-nitrophenol, which produces a yellow color that can be measured spectrophotometrically. Similarly, 4-Nitrophenyl beta-glucoside (pNP-Glc) can be used to assay beta-glucosidase activity. 4-Nitrophenyl beta-globotrioside (pNP-Glo) is a similar compound that has been designed as a substrate for glycosidases that cleave terminal beta-galactosyl-(1-&gt;3)-N-acetylgalactosamine residues. In these assays, the release of 4-nitrophenol can be measured to determine the activity of the enzyme under study. The use of chromogenic substrates like pNP-Glo allows for the rapid and reliable detection of glycosidase activity in a variety of biological samples.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>Please enquire for more information about 1,2,4-trihydroxynaphthalene-4-O-b-D-glucopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Please enquire for more information about 1,2,4-trihydroxynaphthalene-2-O-b-D-glucopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Please enquire for more information about 4-Nitrophenyl valerate including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Methyl 4-nitrophenyl hexylphosphonate is a phosphonate inhibitor of the enzyme serine esterase. It has been shown to be more potent than other irreversible inhibitors such as ethyl 4-nitrophenyl hexylphosphonate and methyl 4-nitrophenoxybutyl phosphate. This compound is an immobilized form of the methyl 4-nitrophenyl hexylphosphonate that is immobilized on an inert surface, such as silica gel, via a covalent bonding process. The immobilization of the molecule ensures that it will not leach from the surface and is stable over time. The molecular weight of this substance is 411.5 Daltons and its molecular formula is C8H15NO4P. This substance has a diameter of 0.8 nm and can be found in crystalline form with a melting point of 143 degrees Celsius at atmospheric pressure. Methyl 4-nitrophenyl</p>

<p>The photorelease of 2-deoxy-2-fluoro-L-fucose from 2-nitrobenzyl-2-deoxy-2-fluoro-L-fucopyranoside was performed under a 365 nm LED illumination. It has been shown in plant cells this liberated 2-deoxy-2-fluoro-L-fucose can be converted by the endogenous metabolic machinery into the corresponding nucleotide-sugar, which then efficiently inhibits Golgi-localized fucosyltransferases.</p>

<p>4-Nitrophenyl-myo-inositol-1-yl phosphate is a highly specific chromogenic substrate used for the detection, quantification, and characterization of phosphoinositide phosphatases. When cleaved by the target enzyme, it generates a highly absorbing 4-nitrophenyl moiety, producing a yellow color that can be measured spectrophotometrically. This versatile substrate is valuable in examining phosphoinositide phosphatase activity, enzyme kinetics, substrate specificity, and inhibition studies in various cellular processes, including signal transduction, lipid metabolism, and membrane trafficking.</p>

<p>Resorufin alpha-D-glucopyranoside is a sensitive fluorogenic substrate that generates a red fluorescent product (resorufin) upon interaction with alpha-glucosidase.</p>

<p>Please enquire for more information about H-Ala-Phe-Pro-pNA trifluoroacetate including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Chromogenic substrate targeting alpha-D-galactosidase</p>