Enzyme Substrates

<p>4-Nitrophenyl 2-acetamido-3,6-di-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-2-deoxy-a-D-galactopyranoside is a fluorogenic substrate that is used in enzyme assays to detect the presence of an enzyme. The substrate reacts with the enzyme to produce a fluorescent product. 4NPAAG can be used for diagnostics, culture media, food testing and environmental testing.</p>

<p>4-Nitrophenyl-myo-inositol-1-yl phosphate is a highly specific chromogenic substrate used for the detection, quantification, and characterization of phosphoinositide phosphatases. When cleaved by the target enzyme, it generates a highly absorbing 4-nitrophenyl moiety, producing a yellow color that can be measured spectrophotometrically. This versatile substrate is valuable in examining phosphoinositide phosphatase activity, enzyme kinetics, substrate specificity, and inhibition studies in various cellular processes, including signal transduction, lipid metabolism, and membrane trafficking.</p>

<p>Bioluminescent substrate for detection of E. coli.</p>

<p>Please enquire for more information about H-Ala-Phe-Pro-pNA trifluoroacetate including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Chromogenic substrate targeting alpha-D-galactosidase</p>

<p>Luciferin-beta-D-glucuronide, dipotassium salt is a fluorescent compound that is used as a diagnostic tool. It can be used to detect the presence of enzymes, such as beta-galactosidase and beta-glucuronidase, in culture media or biological fluids. Luciferin-beta-D-glucuronide, dipotassium salt is also used for food testing and environmental testing.</p>

<p>6-Hexanoylamino-4-methylumbelliferyl b-D-galactopyranoside is a chromogenic substrate for beta-galactosidase.</p>

<p>4-Methylumbelliferyl beta-D-xylobioside is a fluorogenic substrate for xylanase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl beta-D-xylobiosideas a substrate for measuring the xylanase activity is used for detecting bacterial infection and gut microbiome analysis.</p>

<p>Please enquire for more information about 1,8,15,22,29,36-Hexaazacyclodotetracontane-2,7,16,21,30,35-hexone including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>6,8-Difluoro-4-methylumbelliferyl b-D-galactopyranoside, commonly known as DiFMUG, is a fluorogenic substrate to detect beta-galactosidase. It has higher sensitivity than the common MUG (4-methylumbelliferyl beta-D-galactopyranoside) subtrate.</p>

<p>Please enquire for more information about cis-6,7-Dimethyl-5,6,7,8-tetrahydropterin hydrochloride including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>4-Chloro-1-naphthol is a chemical compound that has been used in clinical pathology to identify human immunoglobulin. It is also used as a potential biomarker for the diagnosis of Alzheimer's disease. 4-Chloro-1-naphthol binds to the amyloid fibrils and has anti-angiogenic effects in the hippocampus, which may be due to its ability to inhibit the formation of new blood vessels. This chemical compound can be used as a substrate molecule for electrochemical impedance spectroscopy and is used in wastewater treatment.</p>

<p>Chemiluminescence substrate for AP detection</p>

<p>5-Iodo-3-indolyl b-D-galactopyranoside is a chromogenic substrate used for beta-galactosidase. It is incorporated into media or detection kits for rapid and direct identification of microorganisms.</p>

<p>AquaSpark® Enhanced HRP Detection Solution is a sensitive chemiluminescent substrate solution for detecting horseradish peroxidase (HRP) conjugates on immunoblots (Western blot, dot blot) and in enzyme-linked immunosorbent assays (ELISA). The ready-to-use detection solution contains the AquaSpark® substrate and the oxidizing reagent hydrogen peroxide (H2O2).</p>

<p>Espresso-Gal (Espresso-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields an espresso brown colored precipitate. Espresso-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.</p>

<p>Fluorogenic substrate for β-glucosidase</p>

<p>Chromogenic substrate for alkaline phosphatase</p>

<p>Fluorogenic substrate for β-galactosidase</p>

<p>4-Nitrophenyl-beta-D-glucopyranoside is a fluorescent probe that can be used in the study of the biochemical properties of enzymes. This compound binds to the enzyme's active site, which allows for transfer reactions to occur. 4-Nitrophenyl-beta-D-glucopyranoside is an artificial substrate that is biochemically inert and has a similar physical structure to natural substrates. It can be used as a model system to study enzymatic reactions and has been shown to have pharmacokinetic properties and a pH optimum. The detection sensitivity of this compound is high, making it ideal for use in biochemical studies.</p>