Enzyme Substrates

<p>Aldol® 518 alpha-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of alpha-galactosidase activity. The enzyme substrate is cleaved by alpha-galactosidase positive bacteria and yeasts in liquid or solid media, yielding red color and red fluorescence signal. Aldol® 518 alpha-D-galactopyranoside can also be used to detect alpha-galactosidase (encoded by the Mel1 gene) in yeast gene reporter assays or for identification of alpha-gal positive transformants on agar plates. Aldol® 518 alpha-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>Espresso-Gal (Espresso-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields an espresso brown colored precipitate. Espresso-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.</p>

<p>Please enquire for more information about Ac-D-Cha-GR-aminoluciferin including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>AquaSpark® Enhanced HRP Detection Solution is a sensitive chemiluminescent substrate solution for detecting horseradish peroxidase (HRP) conjugates on immunoblots (Western blot, dot blot) and in enzyme-linked immunosorbent assays (ELISA). The ready-to-use detection solution contains the AquaSpark® substrate and the oxidizing reagent hydrogen peroxide (H2O2).</p>

<p>Chemiluminescence substrate for AP detection</p>

<p>4-Chloro-1-naphthol is a chemical compound that has been used in clinical pathology to identify human immunoglobulin. It is also used as a potential biomarker for the diagnosis of Alzheimer's disease. 4-Chloro-1-naphthol binds to the amyloid fibrils and has anti-angiogenic effects in the hippocampus, which may be due to its ability to inhibit the formation of new blood vessels. This chemical compound can be used as a substrate molecule for electrochemical impedance spectroscopy and is used in wastewater treatment.</p>

<p>AquaSpark® Peroxide Probe is a dioxetane-based substrate for the luminescence detection of hydrogen peroxide and other peroxides in biological samples. AquaSpark® Peroxide Probe is the most sensitive probe for H2O2 detection available. It is also an extremely versatile probe for oxidase detection applicable in a wide range of biocompatible buffers. For example it can be used for glucose oxidase detection and for the quantitation of glucose levels (when coupled to the glucoseoxidase activity in an assay). In addition, AquaSpark® Peroxide Probe is used for the quantification of other biologically relevant compounds (e.g. amino acids) using the corresponding peroxide releasing oxidases.</p>

<p>L-Phenylalanine 7-amido-4-methylcoumarin trifluoroacetate salt is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.</p>

<p>Chemiluminescent phosphatase substrate in a wide pH range (pH 6 - 10)</p>

<p>Aldol® 470 phosphate, disodium salt is a fluorogenic substrate that is used in the detection of enzyme activity. It can be used to detect the presence of an enzyme by measuring the light emitted from the substrate after it has been converted to fluorescent product. Aldol® 470 phosphate, disodium salt is a chromogenic substrate that is used for detecting and measuring enzyme activity. The product of this reaction can be measured by absorbance or fluorescence spectroscopy, making it useful for many different types of diagnostic tests. This product can be conjugated with a variety of ligands for use in diagnostic assays and research. Aldol® 470 phosphate, disodium salt can also be used in culture media as a bioluminescent substrate for bacterial growth and metabolism studies. This product can also be used as an environmental testing reagent or as a staining agent in microscopy applications.</p>

<p>Aldol® 518 alpha-D-glucopyranoside is a fluorogenic enzyme substrate used for detection of alpha-D-glucosidase activity. The colorless enzyme substrate is cleaved by alpha-D-glucosidase, generating red fluorescent signal and in some conditions, an orange colour in the visible spectrum can get formed, staining bacterial colonies in faint orange coloration. Aldol® 518 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>5-Cyano-3-indolyl-b-D-galactopyranoside is a chemiluminescent substrate that can be used to measure the activity of esterases and other enzymes. It has been shown to be a potent inhibitor of human erythrocyte esterase. The compound is also a potent inducer of bioluminescence in bacteria, which can be used for diagnostic applications. 5-Cyano-3-indolyl-b-D-galactopyranoside is a fluorogenic substrate that can be used to measure the activity of phosphatases and other enzymes. This product has an enzyme substrate function, which can be used for enzyme assays. 5CIGP is useful for measuring high levels of enzyme activity in the presence of strong inhibitors such as phenylmethylsulfonyl fluoride (PMSF).</p>

<p>Used for asymmetric ketone reduction into chiral alcohols</p>

<p>Chromogenic substrate targeting cellulases</p>

<p>Chemiluminescence substrate for C2-esterase (acetyl esterase)</p>

<p>5-Bromo-4-chloro-3-indoxyl-1-acetate is a chromogenic substrate used to detect the specific enzymatic activity of esterase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl-1-acetate is used in bacterial esterase activity assays (e.g. Pseudomonas spp.).</p>

<p>Please enquire for more information about 4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>5-Amino-3-indoxyl-b-D-galactopyranoside is a chromogenic substrate for the enzyme galactosidase. 5-Amino-3-indoxyl-b-D-galactopyranoside is used in diagnostic tests to identify the presence of lactose intolerance. It can also be used to detect traces of blood in food, water, and other substances. This product has a high purity that makes it suitable for conjugation applications.</p>

<p>Please enquire for more information about 4-Methylumbelliferyl b-D-xylopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>4-Methylumbelliferyl beta;-D-glucuronide (MUG) is commonly used as a substrate for detecting beta;-glucuronidase. MUG is used for identifying E. coli contamination in food, drinkingwater, clinical samples and for rapid bacterial identification in blood cultures. It is recommended in international normatives (ISO, DIN) as a fluorogenic substrate for detection of E. coli. MUG is also a tool assaying the glucuronidase gene in plants.</p>