Chromogenic Substrates

<p>Chromogenic beta-Glucosidase substrate yielding a yellow solution upon cleavage.</p>

<p>Chromogenic substrate for N-acetyl-β-D-glucosaminidase yielding a blue precipitate. Contained in certain mycological culture media. Used in histochemistry.</p>

<p>6-Bromo-2-naphthyl beta-D-glucuronide is a substrate for beta-glucuronidase, an enzyme involved in detoxification and bacterial metabolism. Upon enzymatic hydrolysis, it releases 6-bromo-2-naphthol, which forms a detectable dye when reacted with diazonium salts. This compound is widely used in microbiology to detecting E. coli in water testing and histochemical staining. 6-Bromo-2-naphthyl beta-D-glucuronide is also been used in cancer research studies. It has been found that some tumors are related to a high activity of beta-glucuronidase, responsible to reactivate the carcinogens and promote tumor progression, developing a drug resistance to chemotherapy.</p>

<p>4-Nitrophenyl b-D-thioglucopyranoside is a highly sensitive chromogenic substrate designed for the colorimetric detection and quantification of thioglucosidase enzymes. Upon enzymatic cleavage, it releases a highly absorbing 4-nitrophenyl moiety, generating a yellow color that can be readily measured spectrophotometrically. This substrate is widely utilized in the study of enzyme kinetics, substrate specificity, and inhibitor screening for thioglucosidases involved in various metabolic pathways and biological processes.</p>

<p>Chromogenic substrate for β-D-galactosidase yielding a precipitate. Mostly used in histochemistry.</p>

<p>1-Naphthyl β-D-mannopyranoside is a chromogenic (also fluorogenic) substrate. After cleavage, 1-naphthol is released and is used to quantify beta-mannosidase activity.</p>

<p>N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine (TMB) sodium salt is a highly sensitive chromogenic substrate used for the detection and quantification of peroxidase enzyme activity. This water-soluble substrate undergoes a color change from colorless to blue upon oxidation by peroxidase enzymes in the presence of hydrogen peroxide. The reaction can be stopped by adding an acid, resulting in a yellow color that can be measured spectrophotometrically. TMB sodium salt is widely used in various applications, including enzyme-linked immunosorbent assays (ELISA), immunohistochemistry, and other diagnostic tests, due to its high sensitivity, low background signal, and excellent stability.</p>

<p>Naphthol AS-BI sulphate potassium salt is a modified naphtol AS substrate used for alkaline and acid phosphatase.</p>

<p>Chromogenic substrate for alkaline and acid phosphatase</p>

<p>3,3'-Diaminobenzidine (DAB) is an organic compound derived from benzidine. DAB is water-soluble as the tetrahydrochloride salt and is used in immunohistochemical staining of nucleic acids and proteins. Within research in Alzheimer's disease, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize it, producing an easily observable brown colour. 3,3’-Diaminobenzidine tetrahydrochloride hydrate is also available as the tetrahydrochloride salt and as the free base.</p>

<p>5-Iodo-3-indolyl-b-D-galactopyranoside-6-sulfate sodium salt is a high purity, water soluble fluorogenic substrate for the detection of beta-galactosidase activity. This product is used in food testing, environmental testing, and diagnostics. 5-Iodo-3-indolyl-b-D-galactopyranoside (5IIAG) is a beta galactosidase substrate that provides a convenient method for determining beta galactosidase activity. This product is also used as an indicator to measure the rate of hydrolysis of ester bonds in other compounds that contain acid functional groups such as glycerides and oils.</p>

<p>4-Nitrophenyl 2-acetamido-3-O-[2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2-deoxyb-D-galactopyranoside is a water soluble, fluorescent probe that can be used for detecting the presence of β-(1→4) -linked galactosaminoglycans. The dye is a substrate for enzymes such as α--mannosidase, β--glucuronidase and β--galactosidase. The dye is also an excellent ligand for lectins such as concanavalin A and wheat germ agglutinin. This product is a diagnostic reagent that has been shown to have high purity and quality. It has CAS No. 93496-53-8 and can be used in food testing, bioluminescence, environmental testing, or high purity applications.</p>

<p>Aldol 458 nonanoate solution (0.75 M) is a substrate for enzymes and ligands, which is used in various applications such as food testing, staining, fluorogenic substrate, environmental testing, chromogenic substrate and conjugate. Aldol 458 nonanoate solution (0.75 M) has high purity and is highly stable at pH 2-12. This product can be used as a chemiluminescence or bioluminescence substrate. The CAS number for this product is 2484873-15-4.</p>

<p>1-Naphthyl a-D-galactopyranoside is a chromogenic substrate use to detect alpha-galactosidase activity.</p>

<p>Ostazin Brilliant Red H-3B is a water soluble, non-toxic, and non-hazardous substrate for enzymes that catalyze reactions involving the cleavage of β-D-glucosides. It is used as a chromogenic or fluorogenic substrate in diagnostic tests. Ostazin Brilliant Red H-3B can also be used in bioluminescence reaction with luciferase to measure ATP levels in living cells.</p>

<p>Aldol® 514 inositol-phosphate is a chromogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms, yielding a red-orange color. The substrate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus. Aldol® 514 inositol-phosphate is suitable for use under aerobic and anaerobic conditions.</p>

<p>Aldol® 515 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by acid and alkaline phosphatases secreted by bacteria in liquid or solid media, yielding intense red color and red fluorescence. Aldol® 515 phosphate can also be used in Western blots and other immunoassay applications with phosphatase-coupled secondary antibodies. Aldol® 515 phosphate can be used under aerobic and anaerobic conditions.</p>

<p>Chromogenic substrate for α-D-Fucosidase yielding a blue precipitate.</p>

<p>This product is a chromogenic substrate that is used in the detection of alkaline phosphatase and other enzymes. It is also used in food testing, staining, and diagnostics. This product has high purity with a CAS number of 125328-84-9.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium is a chromogenic substrate for N-acetylglucosamine-6-sulfatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium is used in different applications such as the Mucopolysaccharidosis IIID (Sanfilippo D) screening</p>