Chromogenic Substrates

4-Nitrophenyl b-D-thiomannopyranoside acts as a substrate for β-mannosidase and thioglycoligase. Upon enzymatic cleavage, these substrates release 4-nitrophenol, a yellow chromophore that can be detected spectrophotometrically at 405 nm, facilitating quantitative measurements of enzymatic activity. Consequently, these substrates are widely used in biochemical research, clinical diagnostics, and drug discovery for evaluating the function of specific enzymes in various biological processes.

4-Nitrophenyl trimethylacetate is a cytosolic proteinase inhibitor. It binds to the active site of trypsin and other enzymes that hydrolyze peptide bonds, thereby inhibiting the activity of these enzymes. 4-Nitrophenyl trimethylacetate has been shown to inhibit soybean trypsin, which has led to its use as a model system for studying enzyme inhibition. The binding site on 4-Nitrophenyl trimethylacetate is competitive with respect to substrate binding but not with respect to the enzyme's catalytic triad. This inhibitor also has a high buffer capacity and can be used in low pH systems.

3-Indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a fluorogenic substrate that reacts with the enzyme β-galactosidase to produce a blue product. This product can be used to diagnose the presence of β-galactosidase in culture media, as well as in food and environmental testing. 3IAG is also a chromogenic substrate that can be used for detection of β-galactosidase activity.

4-Nitrophenyl 2-O-(a-D-mannopyranosyl)-a-D-mannopyranoside is a chromogenic substrate for the enzyme, beta-glucosidase. It is used as a food additive to test for the presence of alpha and beta glucosidases in foods. This product is also commonly used as an enzyme substrate and conjugate in biochemical research and diagnostic applications.

4-Nitrophenyl 2-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-a-D-mannopyranoside is a fluorogenic substrate that is used as a diagnostic tool and can be used to detect the presence of β--glucosidase in the gut. It is also used as a conjugate with enzymes such as peroxidase or alkaline phosphatase for use in enzyme catalyzed reactions. The product has been shown to be active against a variety of bacteria, including Staphylococcus aureus and Escherichia coli. This product has been shown to produce chemiluminescence when incubated with horseradish peroxidase and hydrogen peroxide. This product is light sensitive and should be stored at room temperature away from direct sunlight.

4-Nitrophenyl 2,3-di-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside is a fluorogenic substrate that is used in biochemistry as an enzyme substrate. It is a colorless solid that has high purity and high quality. This product is a ligand that binds to the enzyme beta glucosidase. The conjugate of this product with beta glucosidase can be used in diagnostics for the detection of glucose levels in culture media or food testing. This product also has chromogenic and chemiluminescent properties that are used in environmental testing and staining. CAS No.: 54323-05-8

4-Nitrophenyl lacto-N-tetraoside is a chromogenic substrate for enzymes that cleave lactose-specific linkages, such as beta-galactosidases. Upon enzyme cleavage, the pNP group is released, resulting in a yellow-colored solution that can be spectrophotometrically quantified at 405 nm. This substrate is commonly used in detecting lactose hydrolysis activity, such as in dairy industry for production optimization studies, or in microbiology for bacterial lactose fermentation assays. Its tetrasaccharide structure resembles that of lactose and makes it a specific substrate for lactose-utilizing enzymes. The properties of 4-nitrophenyl lacto-N-tetraoside make it a useful tool in enzyme kinetics studies and screening of new lactose-utilizing microorganisms.

4-Chloro-3-indoxyl-1,3-diacetate is a chromogenic substrate used in the identification of bile acids. It reacts with 4-chlorophenol to form a red dye. This compound is also used as a ligand for metal ions.

5-Bromo-4-chloro-3-indoxyl-a-D-xylopyranoside is a fluorogenic substrate that can be used in food testing and environmental testing. This product is a conjugate of the xylosidase enzyme (EC 3.2.1.37) with 5-bromo-4-chloroindoxyl, which has been shown to have a high affinity for the enzyme's active site and enzymatic activity. The purity of this product is > 95% and the CAS number is 68618-41-5.

4-Nitrophenyl 2-O-(α-D-glucopyranosyl)-α-D-glucopyranoside is a specialized chromogenic pNP substrate featuring dual glucose-linked moieties, designed for accurate detection and quantification of specific glycosidase enzymes. Upon cleavage by target enzymes, this substrate releases 4-Nitrophenol, which can be easily monitored spectrophotometrically as a yellow color change. This advanced substrate offers enhanced specificity and sensitivity in enzyme assays, facilitating cutting-edge research in biochemistry, enzymology, and other related fields.

4-Nitrophenyl 2,4,6-tri-O-acetyl-3-O (2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-a-D-glucopyranoside is a chemiluminescent substrate that can be used for the detection of aminoglycosides in biological fluids. It is a high purity and high quality product with CAS No. 482743. The product has been tested for safety and purity using culture media and environmental testing. This product may be used as a bioluminescence substrate to detect the presence of aminoglycosides in samples such as urine, serum or plasma. 4NTPAGDG is also useful as a fluorogenic substrate for diagnostics and enzyme substrates for food testing.

Aldol® 470 L-pyroglutamic acid amide is a chromogenic and fluorogenic enzyme substrate used for detection of pyrrolidonyl peptidase (PYRase) activity. The colorless enzyme substrate is cleaved by PYRase-producing bacteria grown in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, such as, the surface of cellulose acetate filters, polypropylene tubes or microplates, and polyethylene vials. Aldol® 470 L-pyroglutamic acid amide is suitable for use under aerobic and anaerobic conditions.

2-Nitrophenyl-2,3,4-tri-O-acetyl-b-D-glucuronide methyl ester is a tri-O-acetylated glucuronide substrate commonly used in chromogenic assays to detect the activity of beta-glucuronidase. Upon hydrolysis by the enzyme, the substrate releases 2-nitrophenol, which can be spectrophotometrically. The substrate is widely used in diagnostic tests to assess bacterial and fungal infections, as well as in drug metabolism studies.

Aldol® 515 N-glutaryl-L-phenylalanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-glutamate-phenylalanine (GluPhe)-specific peptidase activity. The colorless enzyme substrate is cleaved the GluPhe-specific peptidase, yielding development of orange-red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 N-glutaryl-L-phenylalanine amide can be used in enzyme assays and live bacterial cultures. Aldol® 515 N-glutaryl-L-phenylalanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.