Otros

<p>Aldol 495 nonanoate is a bioluminescent substrate that can be used to measure the activity of Aldolase, which is an enzyme that catalyzes the conversion of glyceraldehyde-3-phosphate and dihydroxyacetone phosphate to D-glyceraldehyde. This substrate is used in various diagnostic assays, including food testing and staining. Aldol 495 nonanoate was developed by Cymit Quimica Pharmaceuticals and has been patented by EP 2427431 and US 8940909.</p>

<p>Ostazin Brilliant Red H-3B Galactomannan is a chemiluminescent substrate used for the detection of β-1,4-galactomannans. It reacts with the glucose residues on the mannan backbone to produce a red fluorescent product. This product can be detected by spectrophotometry at an excitation wavelength of 395 nm and emission wavelength of 510 nm. The Ostazin Brilliant Red H-3B Galactomannan substrate is useful in detecting Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Group A streptococci, Group B streptococci, Group D streptococci, Group G streptococci, and Group C streptococci.</p>

<p>4-Nitrophenyl decanoate is a chromogenic substrate for lipase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl decanoate is used in different applications such as the Pancreatic insufficiency diagnosis, lipid metabolism studies</p>

<p>Chromogenic substrate targeting beta-L-arabinofuranosidase</p>

<p>8-Hydroxyquinoline b-D-glucopyranoside (8-OHG-glu) is a chromogenic enzyme substrate commonly used to detect the presence of beta-glucosidase in biological samples. When hydrolyzed by beta-glucosidase, 8-OHG-glu produces a yellow-orange color, indicating the presence of the enzyme. This substrate is particularly useful for measuring beta-glucosidase activity in soil and plant samples.</p>

<p>4-Nitrophenyl b-D-thiogalactopyranoside is a specialized chromogenic substrate designed for measuring the activity of thio-β-galactosidase enzymes. Upon cleavage by thio-β-galactosidase, it releases the intensely colored 4-nitrophenolate ion, providing a straightforward, sensitive, and quantitative detection method. This substrate is easily incorporated into enzyme-coupled assays, making it a reliable and convenient choice for research and diagnostic applications. With its rapid color development and high signal-to-background ratio, 4-Nitrophenyl b-D-thiogalactopyranoside is ideal for monitoring enzyme kinetics, substrate specificity evaluation, and inhibitor screening in a range of scientific and biotechnological settings.</p>

<p>5-Bromo-4-chloro-1-methyl-3-indolyl-α-D-glucopyranoside is a fluorescent substrate used for the detection of α-glucuronidase.</p>

<p>Chromogenic substrate for neuraminidase yielding a blue precipitate upon cleavage.</p>

<p>5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-mannosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is used in alpha-mannosidosis diagnosis and other lysosomal storage disorder.</p>

<p>Chromogenic substrate for beta-D-glucosidase, yielding a blue precipitate and contained in a chromogenic culture media for the detection of Enterococci, Streptococci, Klebsiella species, Enterobacter species and Listeria species. It is an indolyl carbohydrate that is the beta-D-glucoside of 3-hydroxy-1H-indole, in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for the presence of the enzyme, beta-glucosidase, which cleaves the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole; this immediately dimerises to give an intensely blue product.</p>

<p>2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid ammonium salt is a chromogenic substrate commonly used in the detection and quantification of sialidase activity. Sialidases are a family of enzymes that hydrolyze sialic acid residues from glycoconjugates. This substrate is used to detect bacterial and viral sialidases, which play an important role in pathogen infectivity. The ammonium salt form of the substrate is preferred for its solubility in water-based assay systems.</p>

<p>Substrate for determining hydrolytic activity of chitinase</p>

<p>Chromogenic substrate for α-D-Fucosidase yielding a blue precipitate.</p>

<p>4-Nitrophenyl 6-O-(alpha-D-glucopyranosyl)-beta-D-glucopyranoside is a chromogenic substrate for beta-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 6-O-(alpha-D-glucopyranosyl)-beta-D-glucopyranoside is used in different applications such as the Cellulose metabolism research, biofuel production</p>

<p>3-Indoxyl sulfate, potassium salt (3ISK) is a chemical compound that is soluble in water. It has been used as a marker for bowel disease, and it has been shown to react with analytical methods such as titration calorimetry and lc-ms/ms. 3ISK can be used to determine the optimum concentration for pharmacological treatment of bowel disease. The metabolic fate of 3ISK has been studied extensively and it is found that 3ISK is metabolized by microbial enzymes in the gut, which may lead to an increased incidence of bowel disease in patients with low levels of microbial metabolism. 3ISK also plays an important physiological function by maintaining mitochondrial membrane potential, which could have implications for pharmacological treatment of bowel disease.</p>

<p>Please enquire for more information about 3,4-Cyclohexenoesculetine phosphate disodium including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>N-Acetyl-5-bromo-3-indoxyl-2,3,4-tri-O-acetyl-beta-D-glucuronic acid methyl ester is a chromogenic substrate that can be used for the detection of bile acids and bilirubin in food testing. It is a conjugate of an acetylated 2,3,4 triacetyl glucuronic acid methyl ester with 5 bromo 3 indoxyl. This product has high purity and high quality.</p>

<p>2-Chloro-4-nitrophenyl b-D-lactoside is a chromogenic enzyme substrate used to study the activity of enzymes. It produces a color when hydrolyzed, which makes it a valuable tool in screening for enzyme activity and studying enzyme kinetics.</p>

<p>5-Bromo-4-chloro-3-indoxyl oleate is a chromogenic substrate used to detect the specific enzymatic activity of lipase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl oleate is used in lipid metabolism disorder screening.</p>

<p>Aldol® 584 is a fluorogenic substrate that can be used for enzymatic and chemical assays. It is an important component of the Cymit Quimica Aldol Reagent Kit and other diagnostic kits. Aldol® 584 is used in immunoassays, such as ELISA, to measure the activity of serine proteases, including cathepsin D, elastase, and leukocyte elastase. This product has a CAS number of 2484873-11-0.</p>

<p>Chromogenic substrate for α-galactosidase yielding a blue precipitate. Used for species differentiation within the family Enterobacteriaceae and differentiation of Bifido bacteria species from Lactobacillus species.</p>

<p>N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine (TMB) sodium salt is a highly sensitive chromogenic substrate used for the detection and quantification of peroxidase enzyme activity. This water-soluble substrate undergoes a color change from colorless to blue upon oxidation by peroxidase enzymes in the presence of hydrogen peroxide. The reaction can be stopped by adding an acid, resulting in a yellow color that can be measured spectrophotometrically. TMB sodium salt is widely used in various applications, including enzyme-linked immunosorbent assays (ELISA), immunohistochemistry, and other diagnostic tests, due to its high sensitivity, low background signal, and excellent stability.</p>

<p>Chromogenic substrate for α-glucosidase</p>

<p>5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt is a chemiluminescent substrate that provides a significant increase in light emission. It has a CAS number of 59820-92-0 and is supplied in high purity and high quality. This product is used for the detection of various biological reactions, including diagnostics, enzyme substrates, food testing, and more.</p>

<p>Water soluble substrate for visualizing alkaline phosphatase activity. Produces a magenta colored insoluble end product that is detected visually, when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry.</p>

<p>For the detection of beta-galactosidase activity</p>

<p>Aldol® 470 nonanoate is a chemiluminescent substrate that can be used as a ligand for the detection of C8/C9-esterases, lipases activity, to result in an orange/green color. Aldol® 470 nonanoate has been shown to be active with horseradish peroxidase in a number of different assay systems. The purity and quality of Aldol® 470 nonanoate has been tested with culture media, environmental testing, bioluminescence, staining, fluorogenic substrate, chromogenic substrate, enzyme substrate, food testing and conjugates.</p>

<p>5-Bromo-4-chloro-3-indoxyl choline phosphate is a synthetic enzyme substrate for detecting phosphatidylcholine-specific phospholipase C.</p>

<p>2-Nitrophenyl b-D-xylobioside is a chromogenic substrate for the enzyme xylanase.</p>

<p>2-Fluoro-4-nitrophenyl b-D-galactopyranoside is a chromogenic substrate in enzymatic assays with β-galactosidase. Commonly abbreviated as PFONPG, it is a hydrophilic compound that can be dissolved in saline and blood samples, which makes it a suitable candidate for diagnostic applications as a gene reporter probe by simple analytic methodologies. 2-Fluoro-4-nitrophenyl b-D-galactopyranoside have been used to detect and monitor the growth of prostate tumor cells.</p>

<p>Chromogenic substrate for beta-D-galactosidase yielding a lapis colored precipitate. 5-Bromo-3-indoxyl-beta-D-galactopyranoside can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside as an alternative to 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal), producing a darker blue color for detection of beta-galactosidase activity in bacterial colonies in a colorimetric assay to detect recombinants (white) from non-recombinants (blue). 5-Bromo-3-indoxyl-beta-D-galactopyranoside is also used in histochemistry to demonstrate beta-galactosidase activity in tissues.</p>

<p>4-Nitrophenyl phosphate disodium salt hexahydrate is a substrate of the hydrolase alkaline phosphatase. The products of the enzymatic reaction are 4-nitrophenol and phosphate.</p>

<p>3,3'-Diaminobenzidine (DAB) is an organic compound derived from benzidine. DAB is water-soluble as the tetrahydrochloride salt and is used in immunohistochemical staining of nucleic acids and proteins. Within research in Alzheimer's disease, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize it, producing an easily observable brown colour. 3,3’-Diaminobenzidine tetrahydrochloride hydrate is also available as the tetrahydrochloride salt and as the free base.</p>

<p>Aldol® 495 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by acid and alkaline phosphatases secreted by bacteria in liquid or solid media, yielding orange color and green fluorescence. Aldol® 495 phosphate can also be used in Western blots and other immunoassay applications with phosphatase-coupled secondary antibodies. Aldol® 495 phosphate can be used under aerobic and anaerobic conditions.</p>

<p>2-Naphthyl-α-D-glucopyranoside is a substrate for α-glucosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.</p>

<p>2-Nitrophenyl beta-L-fucopyranoside is a chromogenic substrate used to detect the enzymatic activity of beta-L-fucosidase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl beta-L-fucopyranoside is used in bacterial fucose metabolism studies to identify and quantify the enzymatic activity in, for example, Bacteroides spp..</p>

<p>2-chloro-4-nitrophenyl-beta-D-cellotetraoside is a chromogenic substrate for measuring cellulase activity and other glycosyl hydrolases. The release of 2-chloro-4-nitrophenol can be detected at 405 nm.</p>

<p>4-Nitrophenyl 6-O-(b-D-galactopyranosyl)-b-D-galactopyranoside is a fluorescent substrate that is used in diagnostics and culture media. It can be used to detect the presence of aminoglycoside antibiotics, such as gentamicin, by using the fluorogenic or chromogenic reaction. 4NP6BG has been shown to bind to the beta subunit of RNA polymerase and inhibit transcription, which can be useful for testing environmental samples for the presence of aminoglycosides.</p>

<p>5-Amino-3-indoxyl-beta-D-galactopyranoside (AIG) is a chromogenic substrate that is used for the detection of beta-galactosidase activity. AIG is also used in food testing, diagnostics, and environmental testing. The detection of beta-galactosidase activity can be done by either using the fluorogenic substrate 4-methylumbelliferyl galactopyranoside or the bioluminescence system of firefly luciferase and luciferin. This product has a CAS number of 110114-03-0 and a molecular weight of 417.5 g/mol.</p>

<p>Aldol 458 nonanoate is a fluorogenic substrate that can be used in the detection of enzyme activity particularly C8/C9-esterases, lipases activity producing a yellow/green-orange color. Aldol 458 nonanoate reacts with enzymes, such as oxidoreductases and hydrolases, to produce light. This product is a conjugate of aldol 458 nonanoate and an enzyme substrate for the detection of these enzymes. It has been shown to be useful in food testing, staining, fluorogenic substrate, environmental testing, chromogenic substrate, conjugate, high purity, enzyme substrate for diagnostics and ligand.</p>

<p>p-Nitrophenylphosphorylcholine is a substrate molecule for enzymes that catalyze phospholipid biosynthesis. It is the most common substrate used to study the enzyme activity of phosphatidylcholine synthase and phospholipase A2 in human serum. The reaction mechanism is not well understood, but it appears to involve autocatalysis and an initial nucleophilic attack by p-nitrophenolate on the carbonyl carbon atom of the choline moiety. p-Nitrophenylphosphorylcholine has been shown to exhibit receptor activity on cells, which may be due to its ability to bind with basic proteins. This compound can also inhibit meiosis in Drosophila melanogaster and increase levels of natriuretic peptides in rats, as well as cause cell lysis and polymerase chain reactions in mammalian cells.</p>

<p>5-Bromo-4-chloro-3-indolyl nonanoate is a fluorogenic substrate that is used in the detection of enzymes. The substrate reacts with various enzymes and produces a fluorescent product, which can be detected using a fluorimeter or luminometer. It has been shown to be active against β-galactosidase, α-chymotrypsin, and β-glucuronidase. This compound may also be used as an enzyme substrate for the detection of bacteria in culture media or for food testing. 5-Bromo-4-chloro-3 indolyl nonanoate is not mutagenic or genotoxic when tested on bacterial cells.</p>

<p>Chromogenic substrate for esterase with C8 activity yielding a blue precipitate upon cleavage.</p>

<p>Nalpha-CBZ-L-Citrulline-p-nitroanilide is a synthetic chromogenic enzyme substrate used in protease enzymatic stutides. After hydrolysis, p-nitroaniline is released, which produces a yellow color detectable spectrophotometrically at 405 nm.</p>

<p>a-Naphthyl phosphate disodium salt hydrate is a highly water-soluble chromogenic substrate used to determine phosphatase activity in research and analytical applications, particularly for staining and differentiating acid and alkaline phosphatases.</p>

<p>Aldol® 515 caprylate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C8 esterases, yielding development of red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 caprylate can be used in enzyme assays and live bacterial cultures. Aldol® 515 caprylate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>4-Nitrophenyl 4,6-ethylidene-?-D-maltoheptaoside is a substrate used in a reference measurement method for ?-amylase activity in the diagnosis of acute pancreatitis, approved by the International Federation of Clinical Chemistry (IFCC). The substrate is hydrolysed by serum ?-amylase into shorter 4-nitrophenylated oligosaccharides and their free counterparts. These reaction products are channeled into an indicator reaction with ?-glucosidase and are degraded into glucose and the chromophore, 4-nitrophenol.</p>

<p>Chromogenic substrate for α-amylase yielding a yellow solution upon cleavage.</p>

<p>Chromogenic substrate for β-D-galactosidase, yielding a blue precipitate upon cleavage.</p>

<p>Isopropyl-beta-D-thiogalactopyranoside (IPTG) is a non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression of lac operon to take place. IPTG is present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.</p>