Enzyme

<p>Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces. Lipases/esterases can be used as versatile tools in hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. The Lipase 02 from the yeast Candida rugosa has a temperature optimum in the 30 - 50 °C range and pH optimum between pH 7 and 8.</p>

<p>Protease enzymes break down proteins and are essential for many biological processes, including digestion, cellular regulation and blood clotting. They are also used in many industrial and biotechnological applications for example in food processing and in detergents.</p>

<p>Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces. Lipases/esterases can be used as versatile tools in hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. The Lipase 03 from the yeast Candida rugosa has a temperature optimum in the 30 - 50 °C range and pH optimum between pH 7 and 8.</p>

<p>Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces. Lipases/esterases can be used as versatile tools in hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. The Lipase 01 from the yeast Candida rugosa has a temperature optimum in the 30 - 50 °C range and pH optimum between pH 7 and 8.</p>

<p>Ribonuclease T1 is an endonuclease enzyme, which is derived from the fungus Aspergillus oryzae. It specifically cleaves single-stranded RNA at the 3' end of guanosine residues, which involves hydrolyzing the phosphodiester bond to produce 3′-phosphomononucleotides and 5′-hydroxylated oligonucleotides. This enzyme’s high specificity and catalytic efficiency make it valuable for various applications.</p>

<p>Carboxypeptidase Y (EC 3.4.16.1) is an exopeptidase enzyme. It hydrolyzes peptide bonds of C-terminal residues and it remains active in the presence of urea at low to moderate concentrations. One unit of the Carboxypeptidase Y will hydrolyze 1.0 μmole of a chromogenic peptide substrate, releasing C-terminal alanine and generating a light-absorbing product. Carboxypeptidase Y has been obtained from yeast S. cerevisiae, has a broad substrate specificity and can therefore be used in sequence analysis of proteins. Carboxypeptidase Y has a temperature optimum in the 20 – 30 °C range and pH optimum between pH 6 and 7.</p>

<p>Formaldehyde dehydrogenase (EC 1.2.1.46) is an enzyme that catalyzes the following reaction: formaldehyde + NAD+ + H2O ⇌ formate + NADH + H+ One unit of formaldehyde dehydrogenase will convert 1.0 µmole of formaldehyde to formic acid per minute at pH 7.5 and 37°C in the presence of NAD+.NAD+ is available here.</p>

<p>Glutathione Reductase, baker's yeast, is an enzyme derived from the yeast species *Saccharomyces cerevisiae*. This enzyme is sourced from baker's yeast, providing a renewable and consistent product for various biochemical applications. Its mode of action involves catalyzing the reduction of glutathione disulfide (GSSG) to the sulfhydryl form glutathione (GSH), using NADPH as an electron donor. This reaction is crucial for maintaining the intracellular redox balance by regenerating GSH, the primary cellular antioxidant.</p>

<p>Cellulose catalase is an enzyme-based product, designed specifically to act as a catalyst in the oxidative processes associated with cellulose applications. It is derived from a microbial source, where bacilli or fungi are employed to produce robust catalase enzymes in a fermentation process. The mode of action involves the catalase enzyme’s ability to facilitate the decomposition of hydrogen peroxide into water and oxygen, thereby reducing oxidative damage during cellulose processing.</p>

<p>Lipase 77 recombinantly expressed in P. pastoris comes in a spray-dried formulation. It has its pH optimum at 4-5. Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces catalyzing hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. Lipase 77 was shown to hydrolyze p-Nitrophenyl esters of butyrate and triglycerides.</p>

<p>α-N-Acetylglucosaminidase (recombinant Human NAGLU Protein),  degrades heparan sulfate by hydrolysis of terminal GlcNAc resides in N-acetyl-alpha-D-glucosaminides of heparan sulfate.Activity is measured by its ability to hydrolyse 4-Nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside EN03208 or EM31027. The specific activity is &gt;900 pmol/min/μg, as measured under the decribed conditions.</p>

<p>The secreted phospholipase A2-IIA (sPLA2-IIA, PLA2, systematic name phosphatidylcholine 2-acylhydrolase; EC 3.1.1.4) is an enzyme that catalyses the hydrolysis of glycerophospholipids at the sn2 position, yielding 1-acylglycerophosphocholine and a fatty acid. One unit of secreted phospholipase A2-IIA will hydrolyze 1.0 μmole of substrate per min under optimal conditions.</p>

<p>Phosphorylase B is an enzymatic protein, specifically an isoform of glycogen phosphorylase, derived from rabbit muscle. This enzyme plays a critical role in glycogen metabolism by catalyzing the phosphorolytic cleavage of α(1→4) glycosidic bonds in glycogen, releasing glucose-1-phosphate. The rabbit muscle source provides a well-studied model due to its high enzyme activity and availability, facilitating in-depth biochemical and structural analysis.</p>

<p>hCAII-IN-1 (7f) inhibits CA II/IX with Kis 1.2 nM/113.6 nM, potential for cancer treatment.</p>

<p>hCAXII-IN-1 selectively inhibits HCA IX/XII, promising for new cancer drug development.</p>

<p>hCAI/II-IN-1 (Compound 3h) is a human carbonic anhydrase I and II (hCA I/II) inhibitor that acts on hCA I (IC50: 0.047 μM) and hCA II (IC50: 0.024 μM).</p>

<p>AHR-15010 is a novel anti-arthritic agent.</p>

<p>Potent hCA inhibitor; targets hCA IX, II, XII, I with Ki: 6.5, 7.1, 72.1, 255.8 nM respectively.</p>

<p>hCAIX-IN-14 is a potent inhibitor of human CA IX with a K i value of 134.8 nM .</p>

<p>hCAII-IN-3 (7e) selectively inhibits CA II/IX with Kis of 124.2/30.5 nM, showing promise for cancer treatment.</p>

<p>hCAIX/XII-IN-4 inhibits CAIX/XII with Ki: 4.5 nM (CAXII), 23.6 nM (CAIX), and &gt;10000 nM (CAI/CAII).</p>

<p>Potent carbonic anhydrase inhibitor VI targets CA II, IX, XII with Ki: 40, 39, 900 nM respectively.</p>

<p>Methazolamide-d6 is a GC/LC-MS standard for measuring methazolamide, a glaucoma drug that lowers eye pressure and fluid, reduces seizures, and combats ROS.</p>

<p>Carbonic anhydrase inhibitor 13 (compound 7) is a potent inhibitor of carbonic anhydrase (CA).</p>

<p>hCAIX-IN-7 (6c) selectively inhibits tumor-related isoform hCAIX (KI: 410.6 nM) and weakly affects hCAI/II (KI: &gt;10000 nM).</p>

<p>CDK2-IN-11 inhibits CDK2 (IC50: 6.4 μM) and targets hCA II, IX, XII (Ki: 23.4-56.3 nM); suited for cancer research.</p>

<p>hCAIX-IN-8, a selective hCAIX inhibitor, IC50: 0.024 μM. Also affects CAII, CAVA (IC50s: 1.99, 1.10 μM), limits cell migration, and induces apoptosis.</p>

<p>hCAIX/XII-IN-6: Oral carbonic anhydrase inhibitor, targets hCA I/II/IV/IX/XII, aids rheumatoid arthritis research. Ki: 6697/2950/4093/4.1/7.7 nM.</p>

<p>hCA IX-IN-2 is a highly potent and selective inhibitor of hCA IX, exhibiting an inhibition constant (K I) value of 32.1 nM and demonstrating anti-proliferative</p>

<p>Tyrosinase-IN-7 is a small-molecule tyrosinase inhibitor (IC50 1.57 μM) that suppresses melanin activity and cell growth with low cytotoxicity.</p>

<p>hCAI/II-IN-2 (2b) inhibits hCA I/II (Ki: 40.97 nM, 15.15 nM) and IX (61.88 nM), fights AMS with anti-hypoxic effects, but has low cellular activity.</p>

<p>Peptidyl-prolyl cis-trans isomerase E, also known as Cyclophilin E, Cyclophilin-33, Rotamase E, CYP33, PPIE, is an enzyme which belongs to the cyclophilin-type</p>

<p>hCAXII-IN-4 is a selective and potent CA XII inhibitor with a Ki value of 6.4 nM for human CA XII.</p>

<p>hCAIX-IN-11 inhibits carbonic anhydrases IX &amp; XII with Ki of 32.7 nM &amp; 623.5 nM, respectively.</p>

<p>DTP348: Oral carbonic anhydrase IX inhibitor &amp; hypoxic cell radiosensitizer with sulfamide &amp; 5-nitroimidazole components.</p>

<p>hCAXII-IN-2 is a potent inhibitor of hCA XII (Ki: 84.2 nM) and hCA IX (Ki: 268.5 nM), with low activity on hCA I/II.</p>

<p>L-693612 HCl is an inhibitor of carbonic anhydrase.</p>

<p>6B and 14g inhibit tumor-associated HCA IX with low nanomolar potency; 6K targets HCA XII. All are potential cancer drug leads.</p>

<p>"hCAI/II-IN-3 (compound 5b) is a potent dual hCA I/II inhibitor with Ki: 51.25nM (I), 13.15nM (II), helps treat AMS."</p>

<p>"hCAIX-IN-10 (6i) selectively inhibits carbonic anhydrase IX/XII (Ki: 61.5/586.8 nM), markers in tumor cells, affecting acid-base balance."</p>

<p>Quinethazone is a thiazide diuretic used to treat hypertension. Common side effects include dizziness, dry mouth, nausea, and low potassium levels.</p>

<p>hCAII-IN-2 inhibits human carbonic anhydrases I, II, IX, XII with Ki values of 261.4, 3.8, 19.6, 45.2 nM respectively.</p>

<p>CDK2-IN-12 (10b), potent CDK2 inhibitor, IC50: 11.6 μM; inhibits hCA I/II/IX/XII, KI: 3534/638.4/44.3/48.8 nM; anti-cancer properties.</p>

<p>L 645151 is an inhibitor of lipophilic CA.</p>

<p>Compound 6q inhibits hCAIX/CAXII; K i : hCAI &gt;10000 nM, hCAII &gt;10000 nM, hCAIX 66.2 nM, hCAXII 4.4 nM.</p>

<p>Disulfamide: Oral carbonic anhydrase inhibitor, IC50 0.07 μM, diuretic by blocking Na+/HCO3- reabsorption.</p>

<p>hCAXII-IN-3 (Compound 6o) is a selective inhibitor of human carbonic anhydrase XII (hCAXII) (Ki: 10.0 nM).</p>

<p>GV2-20 is an effective carbonic anhydrase 2 inhibitors.</p>

<p>LP-533401 is an inhibitor of Tryptophan hydroxylase 1. It is used for regulates serotonin production in the gut.</p>

<p>CA inhibitor 14 blocks hCA I/II/IX/XII (K i of 1203/99.7/9.4/27.7 nM) and CDK2 (IC50: 20.3 μM), showing antitumor effects.</p>

<p>(R)-Nepicastat HCl (RS-25560-198 HCl), R-enantiomer, inhibits bovine/human dopamine-β-hydroxylase (IC50: 25.1/18.3 nM); not affecting other enzymes/receptors.</p>

<p>L-693612 is an inhibitor of carbonic anhydrase.</p>

<p>L 662583 is a topical inhibitor of carbonic anhydrase.</p>

<p>HCAIX-IN-2 (compound 9d) is a selective inhibitor of carbonic anhydrase and acts on hCA IX (Ki: 24.6 nM) and hCA XII (Ki: 45.3 nM).</p>

<p>hCAXII-IN-5, or compound 6o, is a potent, selective hCAXII inhibitor with Ki values: hCAI &amp; II &gt;10000, hCAIX 286.1, hCAXII 1.0 nM.</p>

<p>WES-1 (Compound 8g), a carbonic anhydrase IX inhibitor (Ki: 55.9 μM), exhibits broad-spectrum antiproliferative activity against various cancer cell lines,</p>

<p>Telotristat besilate is an inhibitor of tryptophan hydroxylase.</p>

<p>Hematopoietic Prostaglandin D Synthase (HPGDS) belongs to the GST superfamily and Sigma family.</p>

<p>hCA I-IN-1 inhibits hCA I (Ki: 38.3 nM), II (Ki: 716.4 nM), IX (Ki: 940.1 nM), XII (Ki: 192.8 nM).</p>

<p>Nicotinate-Nucleotide Pyrophosphorylase (QPRT) belongs to the nadC/modD family.</p>

<p>Clofenamide is a low-ceiling sulfonamide diuretic.</p>

<p>Phenylthiazolylthiourea ia a dopamine-beta-hydroxylase inhibitor.</p>

<p>Compound 6a: Selective hCAIX/XII inhibitor; Ki: 30 nM (hCAIX), 3.6 nM (hCAXII); &gt;10,000 nM (hCAI/II).</p>

<p>Expression system: Baculovirus&lt;br&gt;Length: 1-415, Full Length&lt;br&gt;Activity: Not Tested</p>

<p>hCAI/II-IN-4 inhibits hCA I &amp; II (Ki: 16.95 &amp; 15.22 nM), hCA IX (Ki: 27.04 nM), has anti-hypoxia benefits and is low-toxic for AMS research.</p>

<p>FC11409B is a CAIX inhibitor, inhibiting breast cancer invasion and metastasis.</p>

<p>hCA I-IN-2 (6d) inhibits hCA I (Ki: 18.8 nM) more selectively over II, IX, XII (Ki: 375.1, 1721, 283.9 nM).</p>

<p>SU 10603 is a specific inhibitor of 17α-hydroxylase (also known as CYP17A1 and P450c17) used to study steroid hormone synthesis.</p>

<p>hCAIX-IN-5 was a selective hCAIX inhibitor, inhibiting hCAI, hCAII, hCAIV, and hCAIX with Ki values of &gt;10000, &gt;10000, 130.7, and 829.1 nM, respectively.</p>

<p>α-Glucosidase is a glycoside hydrolase enzyme that hydrolyzes α-1,4-linked D-glucose residues to produce α-D-glucose. This enzyme has been isolated from Bacillus stearothermophilus and is used as an industrial catalyst in the production of glucose syrups.  One Unit of α-Glucosidase will release 1.0 µmole of p-nitrophenol from the chromogenic substrate mimic 4-nitrophenyl α-D-glucopyranoside per minute under optimum conditions.</p>

<p>Endo-β-1,4-Mannanase (other names Mannan endo-1,4-β-mannosidase, endo-β-1,4-mannase, β-mannanase B, β-1, 4-mannan 4-mannanohydrolase, endo-β-mannanase, β-D-mannanase, 1,4-β-D-mannan mannanohydrolase; EC 3.2.1.78) is an enzyme, catalyzing the hydrolysis of -1, 4-mannosidic linkages in mannans, glucomannans and galactomannans. One unit of Endo-β-1,4-Mannanase will release 1.0 µmole of mannose reducing-sugar per minute from a 3mg/ml mannan solution at pH 5.5 and 37degC.  Expressed in U/g.</p>

<p>Sultiame is an inhibitor of carbonic anhydrase. Sultiame can be used in antiepileptic research.</p>

<p>Protease enzymes break down proteins and are essential for many biological processes, including digestion, cellular regulation and blood clotting. They are also used in many industrial and biotechnological applications for example in food processing and in detergents.</p>

<p>hCAII-IN-9 inhibits hCA II/IX/XII with IC50s of 1.18, 0.17, 2.99 μM; not BBB permeable.</p>

<p>Endoproteinase Glu-C (Glutamyl endopeptidase, V8 protease, GluV8, EC 3.4.21.19) is a protease that hydrolyzes peptide bonds at the carboxylic side of either exclusively Glu, or Glu and Asp residues, depending on the buffer conditions. One unit of endoproteinase Glu-C will generate 1.0 μmole of p-nitroaniline from Z-Phe-Leu-Glu-pNA peptide mimic substrate per minute at pH 7.8 and 25 °C. Z-Phe-Leu-Glu-pNA substrate is available here.molecular weight ~ 27000.</p>

<p>Carboxypeptidase Y (EC 3.4.16.1) is an exopeptidase enzyme. It hydrolyzes peptide bonds of C-terminal residues and it remains active in the presence of urea at low to moderate concentrations. One unit of the Carboxypeptidase Y will hydrolyze 1.0 μmole of a chromogenic peptide substrate, releasing C-terminal alanine and generating a light-absorbing product.</p>

<p>Acetylcholinesterase is an enzyme that breaks down acetylcholine</p>

<p>Superoxide dismutase is an enzyme that catalyzes the conversion of harmful superoxide into hydrogen peroxide and oxygen.</p>

<p>Bupicomide is a dopamine β-hydroxylase inhibitor with antihypertensive and vasodilatory activity and may be used in the study of hypertension.</p>

<p>75u/mg - Glyceraldehyde 3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) is an enzyme that catalyzes the following reaction:  glyceraldehyde 3-phosphate + NAD+ + Pi ⇌ glycerate 1,3-bisphosphate + NADH + H+  One unit of GAPDH will convert 1.0 μmole of glyceraldehyde 3-phosphate into glycerate 1,3-bisphosphate per minute at pH 8.5 and 37 °C in the presence of NAD+ and phosphate. NAD+ is available here.</p>

<p>Proteinase K is used for the general digestion of proteins and removal of protein contamination in nucleic acids. Addition of Protease K also stabilizes nucleic acids by degrading any nucleases present. Proteinase K is active in wide range of pH range, in the presence of SDS, urea and Guanidinium chloride at low to moderate concentrations. Proteinase K is also known under names of protease K and endopeptidase K.</p>

<p>Maltose phosphorylase (systematic name maltose:phosphate 1-beta-D-glucosyltransferase; EC 2.4.1.8) is an enzyme that catalyzes the following reaction:  maltose + Pi ⇌ D-glucose + beta-D-glucose 1-phosphate  One unit of maltose phosphorylase will produce 1.0 μmole of D-Glucose from maltose per minute at pH 7.0 and 30°C.</p>

<p>E. coli recombinant sialic acid aldolase (EC 4.1.3.3) from Pasteurella multocida.  One unit is defined as the amount of enzyme that catalyses the formation of 1 umol Neu5Ac from ManNAc and Pyruvate per minute at 37 ℃.Activity: 9U/mg</p>

<p>Choline oxidase (EC 1.1.3.17) is an enzyme that catalyzes the following reaction: choline + O2 + H20 ⇌ betaine aldehyde + H2O2One unit of choline oxidase will form 1 μmole of H2O2 by oxidizing choline to betaine aldehyde per min at pH 8.0 and 37 °C. You can remove the build-up of hydrogen peroxide using catalase.</p>

<p>Nucleoside phosphorylase (Purine nucleoside phosphorylase, PNP, PNPase, inosine phosphorylase, inosine-guanosine phosphorylase; EC 2.4.2.1) is an enzyme that catalyzes the following reaction:  purine nucleoside + Pi ⇌ purine + alpha-D-ribose 1-phosphate  One unit of nucleoside phosphorylase will phosphorylate 1.0 micromole of inosine to hypoxanthine and alpha-D-ribose 1-phosphate per min at pH 7.4 and 25°C.</p>

<p>E. coli recombinant α-2,6 sialyltransferase from Neisseria meningitidis. One unit is defined as the amount of enzyme that catalyses the formation of 1 μmol CMP-Neu5Ac from CTP and Neu5Ac per minute at 37 ºC.Activity: 100U/mg</p>

<p>Glucosidases are enzymes belonging to the family of oxidoreductases. They catalyse the hydrolysis of starches to simple sugars. Glucosidase is widely used in the food, carbohydrate and biofuels industries. In recent years, its applicability has expanded to biotechnology for its potential application in bioenzymatic fuel cells.</p>

<p>Urokinase (urokinase-type plasminogen activator, uPA; EC 3.4.21.73) is as serine protease. Its physiological substrate is plasminogen. Urokinase converts plasminogen into an active enzyme, plasmin, which is also a serine protease. In its active form plasmin plays an important role in dissolving blood clots. Despite its name, Urokinase is not a kinase.</p>

<p>hCAI/II-IN-6 is a selective and orally active inhibitor of human carbonic anhydrase (CA).</p>

<p>beta-Galactosidase (EC 3.2.1.23, shortly beta-Gal, also know as lactase) catalyses the hydrolysis of beta-d-galactoside in the presence of water to galactose and alcohol, or lactose into glucose and galactose. beta-Gal has a molecular weight of 540,000 and is composed of four identical subunits of MW 135,000, each with an independent active site. The enzyme has divalent metals as cofactors, with chelated Mg2+ ions required to maintain active site conformation. The molecule contains numerous sulfhydryl groups and is glycosylated.</p>

<p>A proteolytic enzyme synthesized by the HIV cell as part of the GagPol polyprotein</p>

<p>Cocarboxylase hydrochloride is a coenzyme derivative, which is primarily sourced from thiamine (vitamin B1). It plays a crucial role in biochemical processes by facilitating the enzymatic decarboxylation of alpha-keto acids within the cellular environment. This action is fundamental in energy production as it aids in the conversion of pyruvate to acetyl-CoA, subsequently entering the citric acid cycle. Cocarboxylase hydrochloride’s involvement in carbohydrate metabolism is especially vital for tissues with high metabolic rates, such as the heart and brain.</p>

<p>D-Alanine aminotransferase (L-glutamic-pyruvic transaminase; EC 2.6.1.21) is an enzyme that catalyzes the following reaction:  D-alanine + α-ketoglutarate ⇌ pyruvate + D-glutamate  Please enquire for more information about D-Alanine Aminotransferase, Bacilus subtilis, Recombinant including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Butyrylcholinesterase is an enzyme made in the liver and found mainly in blood plasma. Butyrylcholinesterase (EC 3.1.1.8), also known as BChE or BuChE, is a nonspecific cholinesterase enzyme that hydrolyses choline-based esters. One unit of Butyrylcholinesterase will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per minute at pH 8.0 and 37 °C.</p>

<p>Tyrosinase-IN-2, a potent tyrosinase inhibitor, may help in skin lightening and food preservation research.</p>

<p>From pigeon breast muscle - Carnitine acetyltransferase (EC 2.3.1.7, also Carnitine O-acetyltransferase) is an enzyme that catalyzes the following chemical reaction: acetyl-CoA + carnitine ⇌ CoA + acetylcarnitine</p>

<p>DNase I (Deoxyribonuclease I, EC 3.1.21.1) is an endonuclease that cleaves DNA, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3'. On average it produces tetranucleotides. One unit of the DNase I will increase the absorbance of 260nm light at a rate of 0.001/minute in 1 ml reaction volume at 25°C.</p>

<p>Invertase is an enzyme that catalyzes the hydrolysis of sucrose to glucose and fructose and can be found in plants and microorganisms</p>

<p>Glucose Dehydrogenase is an enzyme, which is typically derived from microbial sources such as bacteria and fungi. It functions by catalyzing the oxidation of glucose to gluconolactone, concurrently reducing a cofactor such as NAD⁺ or PQQ. This biochemical reaction is critical in various analytical applications due to its specificity and efficiency in glucose detection.Glucose Dehydrogenase is widely employed in the development of biosensors and diagnostic assays. Its primary application is in blood glucose monitoring devices, where its ability to accurately quantify glucose levels is crucial for managing diabetes. Additionally, it is utilized in research and development settings for biochemical assays that require precise glucose measurements. The enzyme's rapid and specific action on glucose molecules makes it an indispensable tool in both clinical and laboratory environments, contributing to advancements in biosensing technologies and metabolic studies.</p>

<p>Lysozyme is a bacteriolytic enzyme, which is primarily derived from hen egg whites. It functions by hydrolyzing the β-1,4-glycosidic linkages in the peptidoglycan layer of bacterial cell walls, particularly in Gram-positive bacteria. This enzymatic activity results in the lysis and subsequent death of the bacterial cells, providing a potent antimicrobial effect.</p>