- Furin Substrate
Internally quenched fluorogenic peptide substrate containing anthranilic acid as fluorescent donor and m-nitro-tyrosine as acceptor (quencher). The sequence Abz-RVKRGLAY(NO2)E is based on the sequence of hemagglutinin. The FRET substrate is efficiently cleaved by furin, a subtilisin-like eukaryotic serine endoprotease with Km = 3.8 uM and kcat = 29.3 s-1 (kcat/Km = 7’710’000 M-1s-1). Its kcat/Km value is over 2000-fold higher than that of the commonly used substrate Boc-Arg-Val-Arg-Arg-AMC (I-1645). FRET Substrate for the basic residue-specific yeast aspartyl protease yapsin 1.
Technical inquiry about: 01-4026550 Abz-Arg-Val-Lys-Arg-Gly-Leu-Ala-m-nitro-Tyr-Asp-OH
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