01-I-1140 - z-gly-gly-arg-amc-hcl | 102601-58-1

Z-GGR-AMC has been used as substrate in a direct fluorometric assay of urokinase and tissue-type plasminogen activator as well as for assaying trypsin and thrombin.The substrate is used as thrombin substrate in the calibrated automated thrombogram (CAT), an automated test developed by Hemker et al., for determining hyper- and hypocoagulability. Z-GGR-AMC can also be used for determining the tryptic activity of proteasomes.

<p>Z-Gly-Gly-Arg-AMC HCl</p>

<p>Z-Gly-Gly-Arg-AMC·HCl is a proteolytic enzyme that cleaves proteins at the carboxyl side of the amino acid arginine. It has been shown to have potential as a drug target and has been found to be active against carcinoma cell lines, but not normal cells. Z-Gly-Gly-Arg-AMC·HCl is activated by light and can be inhibited by natural compounds such as 2-aminoethoxydiphenyl borate. In addition, it specifically cleaves proteins at the carboxyl side of arginine residues, which makes it useful for studying protein degradation mechanisms in living cells and tissues.</p>

<p>Applications N-CBZ-Glycyl-glycyl-L-arginine 7-Amido-4-methylcoumarin Hydrochloride is a fluorogenic substrate for uPA (urokinase).<br>References Zimmerman, M., et al. Proc. Natl. Acad. Sci. USA 75, 750 (1978);<br></p>