5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt trihydrate

CAS: 370100-64-4

Ref. 3D-B-7400

Product Information

• Name: 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt trihydrate
• Synonyms: X-beta-D-glucuronide sodium saltEB03171
• Brand: Biosynth
• Description: Chromogenic substrate for beta-glucuronidase (GUS) yielding blue precipitate upon cleavage. Used for identification of beta-glucuronidase. X-beta-D-glucuronide has been reported as chromogenic substrate in a number of chromogenic E. coli/ coliform culture media. About 95% of common E. coli possess beta-D-glucuronidase activity. Such chromogenic culture media are used for detection and enumeration of E. coli in food samples such as meat, dairy products and shellfish and have clinical applications in the assessment of urinary infections. It has gained international acceptance as highly accurate test for the presence of E. coli in drinking water samples. It is also used for the detection of the expression of GUS (E. coli beta-glucuronidase) gene fusion marker constructs in plants. The sodium salt has advantages over the cyclohexylammonium salt as being non-electrostatic and possessing a naturally-occurring cation.

Chemical properties

• Molecular weight: 498.65 g/mol
• Formula: C₁₄H₁₈BrClNNaO₁₀
• Purity: Min. 99.0 Area-%