SARS-CoV-2 Spike Receptor-Binding Domain (RBD) Mouse Monoclonal Antibody

Ref. 3D-BF1354

Product Information

• Name: SARS-CoV-2 Spike Receptor-Binding Domain (RBD) Mouse Monoclonal Antibody
• Brand: Biosynth
• Description: Mouse monoclonal antibody to SARS-CoV-2 spike receptor binding domain (RBD), purified by protein A chromatography in PBS, pH, 7.4.The Coronavirus Disease-2019 (COVID-19), associated with symptoms such as shortness of breath, fever and muscle ache, is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), whose emergence was first detected in December 2019. This mouse Mab product is complementary to the RBD located on the S1 subunit of the SARs-CoV-2 spike protein which works alongside the S2 subunit of the spike protein to assist in the virus’ entry into the host cells. S1 binds to host angiotensin-converting enzyme 2 (ACE2) receptors located in the host’s organs, namely in the arterial and smooth muscles cells, endothelial cells and in venous endothelial cells. The SARS-CoV-2 can then fuse to the host membrane through S2’s formation of a hairpin structure. Specifically a receptor-binding motif (amino acids 438-506) located on the RBD interacts with the second ACE2 peptidase (a furin-like protease) which cleaves the amino acids 682-685 in-between S1 and S2 subunits. The receptor binding domain is of particular interest for the development of neutralizing antibodies and it can, alongside other spike protein components, be used as an immunogen in vaccine development. Potential applications of this product are in sandwich immunoassays where it can be used as a detection antibody conjugated with HRP, or as either a capture antibody or a detection antibody conjugated with Biotin.