3x FLAG peptide

Ref. 3D-CRB1001528

Product Information

• Name: 3x FLAG peptide
• Synonyms: H-DYKDDDDKGDYKDDDDKGDYKDDDDK-OHDYKDDDDKGDYKDDDDKGDYKDDDDK-acidH-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Gly-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Ly s-Gly-Asp-Tyr-Lys-Asp-Asp-Asp-As p-Lys-Ala-Cys-Ile-Asp-OH
• Brand: Biosynth
• Description: The synthetic canonical Flag sequence has been shown to be most effective with the Asp-Tyr-Lys-Xaa-Xaa-Asp motif triplicated for applications in protein analysis followed by the eight amino acids at the C-terminus of the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys). Due to the hydrophilic nature of the peptide the Flag tag typically resides on the surface of the recombinant protein thus minimising any effects on the function or transport of the fusion protein. The tag can be used in conjunction with other tags such as HA or myc depending on the application. FLAG is an artificial antigen to which high affinity monoclonal antibodies have been raised, therefore allowing for highly effective protein purification by affinity chromatography as well as accurate localisation of FLAG tagged proteins within living cells, or Western blots. FLAG peptide can be used to effectively purify complexes with multiple proteins as its mild purification procedure tends not to disrupt such complexes. It can be used to obtain proteins of sufficient purity for x-ray crystallography. The 3 x Flag peptide provides powerful detection and purification of recombinant proteins that has been characterised in numerous applications including affinity chromatography, binding assays and structural analysis.

Chemical properties

• Molecular weight: 3,649.8 g/mol