Anti-WT1 antibody - 3mg/mL

Ref. 3D-CRB2005277

Product Information

• Name: Anti-WT1 antibody - 3mg/mL
• Brand: Biosynth
• Description: Wilms tumour 1 (WT1) is a zinc finger transcription factor that plays an important role in the development of several mesodermal tissues including: kidneys; gonads; coronary vasculature; cells within the liver; part of the intestine, and adipose tissue. WT1 is expressed during development, with levels within the developing kidney seen to increase as development proceeds. Postnatally WT1 expression is down-regulated, but upregulated in response to tissue damage in both mice and zebrafish. WT1 has an early role in kidney development and a later role in maintaining kidney function. Mutations in WT1 lead to Wilms tumour, a paediatric kidney cancer, as well as developmental anomalies in the urogenital tract. In zebrafish, two paralogous wt1 genes exist, wt1a and wt1b. The wt1 genes are expressed in a similar and overlapping but not identical pattern. This antibody is raised using an HPLC-purified peptide from a zinc finger domain of zebrafish WT1, an epitope found in both WT1a and WT1b.3mg/ml. Used for ELISA (1:1000) and IHC (1:25000). Reacts with Danio rerio (Zebrafish).Image provided courtesy of Dr. Elizabeth Patton Laboratory. University of Edinburgh: Notochord injury triggers local and sustained wt1b expression. Immunohistochemistry of the injured area with anti-GFP and anti-WT1 antibodies. n > 10; experimental replicates = 5. Scale bar: 20mm. dpf = days post fertilization; hpi = hours post injury; H and E = haematoxylin and eosin.

Chemical properties

• Purity: Min. 95%