HIV-1 gp120 Sheep Polyclonal Antibody, Affinity Purified
Ref. 3D-D7324
| 2mg | To inquire |
Product Information
This polyclonal antibody product: affinity purified Sheep Anti-HIV-1-gp120 was produced by first immunising sheep with a single synthetic peptide which has the amino acid sequence: APTKAKRRVVQREKR. This amino acid sequence corresponds to amino acid section 497-511 in the envelope gene gp120 protein of the BH-10 strain of HIV-1. The antibodies are then isolated from the sheep hyperimmune serum by affinity chromatography using the APTKAKRRVVQREKR sequenced synthetic peptide coupled to Sepharose. The serological activity of the antibodies is checked by ELISA and lyophilised in PBS. 0.15M NaCl (pH 7.4) without preservative.
The glycoprotein gp120 is an essential Human Immunodeficiency virus (HIV) envelope subunit which facilitates the entry of the HIV into CD4 T cells through binding to CD4 receptors and CCR5 or CXCR4 chemokine co-receptors on host cells. This attachment enables the second key envelope glycoprotein gp41 to form a six-helix bundle and therefore fuse to the host cell membrane. This HIV gp120 complementary antibody can be used to detect the presence of the HIV gp120 subunit in antigen detection assays such as ELISA, automated immunoassays, western blot and lateral flow.
Chemical properties
Technical inquiry about: 3D-D7324 HIV-1 gp120 Sheep Polyclonal Antibody, Affinity Purified
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