NHS-Biotin

CAS: 35013-72-0

Ref. 3D-DPG-5707

Product Information

• Name: NHS-Biotin
• Synonyms: (+)-Biotinyl-N-hydroxysuccinimide, (N-Hydroxysuccinimidyl)biotin, 1-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}oxy)pyrrolidine-2,5-dione, 1-{[5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl]oxy}pyrrolidine-2,5-dione, 1H-Thieno[3,4-d]imidazole, 2,5-pyrrolidinedione deriv., 1H-Thieno[3,4-d]imidazole-4-pentanoic acid, hexahydro-2-oxo-, 2,5-dioxo-1-pyrrolidinyl ester, (3aS,4S,6aR)-, 2,5-Pyrrolidinedione, 1-[[5-(hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl)-1-oxopentyl]oxy]-, [3aS-(3aα,4β,6aα)]-, 2,5-Pyrrolidinedione, 1-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]oxy]-, <span class="text-smallcaps">D</span>-Biotin N-hydroxysuccinimide ester, Biotin N-hydroxysuccinimide, Biotin N-hydroxysuccinimidyl ester, Biotin NHS ester, Biotin succinimidyl ester, Biotin-Nhs, Biotin-OSu, EZ-Link NHS-Biotin, Iotin-Nhs, N-Hydroxysuccinimidobiotin, N-Succinimidyl <span class="text-smallcaps">D</span>-biotinate, NHS Biotin, NSC 345668
• CAS: 35013-72-0

Description

NHS-Biotin is a biotin analog that can be used as an affinity label for the purification of specific proteins. NHS-Biotin is synthesized by reacting monoclonal antibody with lysine residues and a hydroxyl group, which form a covalent bond known as an amide linkage. The NHS-Biotin can then be covalently attached to the protein of interest. This attachment allows the protein to be purified by passing it over a column of immobilized streptavidin. NHS-Biotin has been shown to bind to actin filaments in humans and uptake peptide hormones in the bloodstream. It was also found that human serum contains chemical reactions with NHS-Biotin, forming a disulfide bond between two cysteine residues on the protein.

• Brand: Biosynth

Chemical properties

• Molecular weight: 660.64 g/mol
• Formula: C₂₈H₃₉F₃N₆O₉
• Purity: Min. 95%