4-Methylumbelliferyl 2-acetamido-3-O-(b-D-galactopyranosyl)-a-D-galactopyranoside

CAS: 210357-36-1

Ref. 3D-EM03221

Product Information

• Name: 4-Methylumbelliferyl 2-acetamido-3-O-(b-D-galactopyranosyl)-a-D-galactopyranoside
• Synonyms: Gal-b-1,3-GalNAc-a-4MU
• Brand: Biosynth
• Description: 4-Methylumbelliferyl 2-acetamido-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside is a fluorogenic substrate for alpha-N-acetylgalactosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranosideas a substrate for measuring the alpha-N-acetylgalactosaminidase activity is used for Fabry disease diagnosis and Schindler disease screening.

Chemical properties

• Molecular weight: 541.5 g/mol
• Formula: C₂₄H₃₁NO₁₃
• Purity: Min. 95%
• Color/Form: White Powder