4-Methylumbelliferyl 2-acetamido-2-deoxy-3-O-(a-L-fucopyranosyl)-b-D-glucopyranoside

CAS: 383160-12-1

Ref. 3D-EM04792

Product Information

• Name: 4-Methylumbelliferyl 2-acetamido-2-deoxy-3-O-(a-L-fucopyranosyl)-b-D-glucopyranoside
• Synonyms: Fuc-a-1,3-GlcNAc-b-4MU
• Brand: Biosynth
• Description: 4-Methylumbelliferyl 2-acetamido-2-deoxy-3-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-3-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis (deficiency of the enzyme alpha-L-fucosidase) and lysosomal storage disorder screening.

Chemical properties

• Molecular weight: 525.5 g/mol
• Formula: C₂₄H₃₁NO₁₂
• Purity: Min. 95 Area-%
• Color/Form: White Powder