4-Methylumbelliferyl N,N',N''-triacetyl-b-D-chitotrioside

CAS: 53643-13-3

Ref. 3D-EM05139

Product Information

• Name: 4-Methylumbelliferyl N,N',N''-triacetyl-b-D-chitotrioside
• Brand: Biosynth
• Description: 4-Methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotrioside is a fluorogenic substrate for chitinase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotriosideas a substrate for measuring the chitinase activity is used for fungal infection assays.

Chemical properties

• Molecular weight: 785.75 g/mol
• Formula: C₃₄H₄₇N₃O₁₈
• Purity: Min. 95.0 Area-%
• Color/Form: White Off-White Powder