4-Nitrophenyl 3-O-(a-D-glucopyranosyl)-a-D-glucopyranoside

CAS: 136632-95-6

Ref. 3D-EN04813

Product Information

• Name: 4-Nitrophenyl 3-O-(a-D-glucopyranosyl)-a-D-glucopyranoside
• Synonyms: 4-Nitrophenyl a-nigeroside
• Brand: Biosynth
• Description: 4-Nitrophenyl 3-O-(a-D-glucopyranosyl)-a-D-glucopyranoside (pNP-Glc-Glc) is a highly sensitive and potent chromogenic substrate for the enzymatic detection and measurement of alpha-glucosidase activity. The rapid color change triggered by the hydrolysis of pNP-Glc-Glc provides a clear and efficient method for monitoring enzyme kinetics, screening inhibitors, and functional characterization of α-glucosidases. This substrate is soluble in aqueous and polar organic solvents and yields a detectable 4-nitrophenol product with a distinct yellow color under both visible and ultraviolet light, enabling quantitative spectrophotometric measurements. With a superior signal-to-noise ratio and maximal sensitivity, pNP-Glc-Glc is a reliable and robust option for biochemical and biomedical research involving glucosidase enzymes.

Chemical properties

• Molecular weight: 463.39 g/mol
• Purity: Min. 95%
• Color/Form: Powder