4-Nitrophenyl b-D-thiogalactopyranoside

CAS: 1230-27-9

Ref. 3D-EN04995

Product Information

• Name: 4-Nitrophenyl b-D-thiogalactopyranoside
• Synonyms: PNP-1-thio-b-D-Gal
• Brand: Biosynth
• Description: 4-Nitrophenyl b-D-thiogalactopyranoside is a specialized chromogenic substrate designed for measuring the activity of thio-β-galactosidase enzymes. Upon cleavage by thio-β-galactosidase, it releases the intensely colored 4-nitrophenolate ion, providing a straightforward, sensitive, and quantitative detection method. This substrate is easily incorporated into enzyme-coupled assays, making it a reliable and convenient choice for research and diagnostic applications. With its rapid color development and high signal-to-background ratio, 4-Nitrophenyl b-D-thiogalactopyranoside is ideal for monitoring enzyme kinetics, substrate specificity evaluation, and inhibitor screening in a range of scientific and biotechnological settings.

Chemical properties

• Molecular weight: 317.32 g/mol
• Formula: C₁₂H₁₅NO₇S
• Purity: Min. 98 Area-%
• Color/Form: Powder