T4 DNA Ligase (recombinant)

Ref. 3D-ENZ-286

Product Information

• Name: T4 DNA Ligase (recombinant)
• Synonyms: DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.
• Brand: Biosynth
• Description: T4 DNA Ligase is a recombinant protein that catalyzes the formation of DNA molecules from complementary single-stranded DNA. T4 DNA Ligase is used as a research tool in cell biology and biochemistry to study the interactions between proteins and peptides. It is also used to produce recombinant proteins, such as antibodies. T4 DNA Ligase binds to its substrate by an ionic interaction with adenosine triphosphate (ATP) and to the complementary strand by hydrogen bonding. The enzyme cleaves the phosphate backbone of both strands at the junction point, forming two new phosphodiester bonds. This enzyme has been shown to bind to other proteins and peptides, such as receptor tyrosine kinases, which are involved in signal transduction pathways that regulate cell growth and differentiation.

Chemical properties

• Purity: Min. 95%