Product Information
- Resorufin cellobioside
Resorufin b-D-cellobioside is a fluorescent probe that is used to study the biosynthesis of resorufin. It binds to DNA in an acidic environment and fluoresces when excited with UV light. Resorufin b-D-cellobioside has been used to measure the activity of cellulase enzymes in plant cells and as a substrate for binding assays. The enzyme cellulase hydrolyzes this substrate into two molecules of resorufin, which are measured by their absorbance at 520 nm. This fluorescent probe has also been used to isolate bacterial species from life cycles and environmental samples.
Cellulase activity is optimized in an acidic environment with a pH between 3 and 5, where it can bind more readily to dna binding protein. Cellulase enzymes that are active at these pH levels are insoluble, whereas those that are active in alkaline conditions are soluble.
Chemical properties
Technical inquiry about: 3D-ER28833 Resorufin b-D-cellobioside
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