Acridine orange base

CAS: 494-38-2

Ref. 3D-FA39736

Product Information

• Name: Acridine orange base
• Brand: Biosynth
• Description: Acridine orange is a fluorescent dye that can pass through the cell membrane and bind to the cytoplasmic structures of cells. It can be used as a probe for determining the presence of cell nuclei, mitochondria, and other organelles. Acridine orange is also used in experiments to determine mitochondrial membrane potential. In this type of experiment, acridine orange is added to cells and its uptake into mitochondria is observed. The dye changes color from orange to green when it binds with the mitochondria because it emits green fluorescence at higher excitation wavelengths than red fluorescence. Acridine orange has been shown to increase fatty acid synthesis by binding with fatty acid synthase, which is an enzyme involved in fatty acid biosynthesis. Acridine orange also increases ATP levels by inhibiting oxidative phosphorylation in mitochondria.

Chemical properties

• Molecular weight: 265.35 g/mol
• Formula: C₁₇H₁₉N₃
• Color/Form: Orange Powder
• InChI: InChI=1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3
• InChI key: InChIKey=DPKHZNPWBDQZCN-UHFFFAOYSA-N
• SMILES: CN(C)c1ccc2cc3ccc(N(C)C)cc3nc2c1