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5-Carboxyfluorescein
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5-Carboxyfluorescein

CAS: 76823-03-5

Ref. 3D-FC19773

1g
337.00 €
2g
470.00 €
100mg
136.00 €
250mg
156.00 €
500mg
225.00 €
Estimated delivery in United States, on Thursday 16 Jan 2025

Product Information

Name:
5-Carboxyfluorescein
Synonyms:
  • 3',6'-Dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid5-FAM
  • 3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
  • 3′,6′-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylic acid
  • 3′,6′-Dihydroxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylic acid
  • 3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid
  • 5-Fam
  • Carboxyfluorescein,5-
  • FAM (dye)
  • Fam 494/520
  • Flamma 456 Carboxylic acid
  • See more synonyms
  • Hydroxyfluorescein
  • Spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid, 3′,6′-dihydroxy-3-oxo-
Description:

5-Carboxyfluorescein (5-FAM) is a self-quenching dye and one of the most common labelling agents for biomolecules, cell staining and localisation studies. The fluorescence is pH dependent, therefore it has been used to measure the internal pH of cells. As it is only membrane permeable to dead cells, it can be used as a staining agent to distinguish viable and non-viable cells. It is also used in PCR to label the 5’ end of oligonucleotides in the presence of a quencher at the 3’ end (dual labelled probe). During the amplification process, the dye is cleaved and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. The carboxylic acid can be activated to react with a primary amine.

Notice:
Our products are intended for lab use only. For any other use, please contact us.
Brand:
Biosynth
Long term storage:
Notes:

Chemical properties

Molecular weight:
376.32 g/mol
Formula:
C21H12O7
Purity:
Min. 98 Area-%
Color/Form:
Yellow Powder
MDL:
Melting point:
Boiling point:
Flash point:
Density:
Concentration:
EINECS:
Merck:
HS code:

Hazard Info

UN Number:
EQ:
Class:
H Statements:
P Statements:
Forbidden to fly:
Hazard Info:
Packing Group:
LQ:

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