3,3'-Diaminobenzidine tetrahydrochloride hydrate

CAS: 868272-85-9

Ref. 3D-FD31267

Product Information

• Name: 3,3'-Diaminobenzidine tetrahydrochloride hydrate
• Brand: Biosynth
• Description: 3,3'-Diaminobenzidine (DAB) is an organic compound derived from benzidine. DAB is water-soluble as the tetrahydrochloride salt and is used in immunohistochemical staining of nucleic acids and proteins. Within research in Alzheimer's disease, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize it, producing an easily observable brown colour. 3,3’-Diaminobenzidine tetrahydrochloride hydrate is also available as the tetrahydrochloride salt and as the free base.

Chemical properties

• Molecular weight: 360.11 g/mol
• Formula: C₁₂H₁₄N₄·4HCl·xH₂O
• Purity: Min. 97 Area-%
• Color/Form: Powder