3,3',5,5'-Tetramethylbenzidine

Ref. 3D-FT05799

Product Information

• Name: 3,3',5,5'-Tetramethylbenzidine
• Synonyms: TMB3,3',5,5'-Tetramethylbenzidine
• Brand: Biosynth
• Description: TMB is commonly used as a chromogenic substrate for horse radish peroxidase for detection purposes in assay formats, such as enzyme-linked immunosorbent assays (ELISAs). TMB is a colourless solution that changes to blue upon oxidation. In the ELISA format, a stopping solution (such as sulfuric acid) is typically added to a TMB-containing solution to prevent extensive colour generation. After addition of the stop solution, the TMB solution changes from blue through to yellow (450 nm), generating a product that yields a high molar absorptivity. TMB chromogenic substrate is selected for its high sensitivity and stability.

Chemical properties

• Molecular weight: 240.34 g/mol
• Formula: C₁₆H₂₀N₂
• Purity: Min. 99.0 Area-%
• Color/Form: White Powder