Luminol, for Western Blot and ELISA

CAS: 521-31-3

Ref. 3D-L-8600

Product Information

• Name: Luminol, for Western Blot and ELISA
• Synonyms: 5-Amino-2,3-dihydro-1,4-phthalazinedione 3-Amino-phthalhydrazide
• Brand: Biosynth
• Description: Luminol, for Western Blot and ELISA Horseradish peroxidase (HRP), a common marker enzyme for antibodies, catalyses the chemiluminescent oxidation of luminol in the presence of hydrogen peroxide. This transformation is used in ultra-sensitive immunoassays in Western Blot and ELISA. Luminescence can be detected at very low levels using sensitive scintillation counters. Cymit Quimica’s manufacturing process for luminol (L-8600) ensures that the highest purity and lot-to-lot consistency are achieved, making it the product of choice for manufacturers of high-end immunological assays. Performance above-average has also been reported for the application of the product in Western blot procedures. Luminol is also routinely used for the detection of bloodstains in criminal investigation. If the mixture comes in contact with hemoglobin, the iron in the hemoglobin accelerates chemiluminescent oxidation of luminol. Please see Luminol (Cat. number L-8601) for more details.

Chemical properties

• Molecular weight: 177.16 g/mol
• Formula: C₈H₇N₃O₂