3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate
CAS: 207738-08-7
Ref. 3D-T-2150
| 2g | To inquire | ||
| 10g | To inquire | ||
| 25g | To inquire | ||
| 50g | To inquire | ||
| 100g | To inquire |
Product Information
- TMB dihydrochloride
HRP and TMB - it takes two to tango! The enzyme horseradish peroxidase (HRP) has been isolated from the roots of the plant in commercially useful quantities since the nineteen-sixties. Today, HRP-coupled antibodies have become indispensable in immunoassays. The most popular chromogenic substrate for HRP is Tetramethylbenzidine (TMB), in particular in enzyme-linked Immunosorbent assays (ELISA). HRP converts TMB into a blue color that can be read spectrophotometrically at 650 nm. The HRP reaction can be stopped by adding sulfuric or phosphoric acid. This yields a yellow color that can be read at 450 nm.
Chemical properties
Technical inquiry about: 3D-T-2150 3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate
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