3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate

CAS: 207738-08-7

Ref. 3D-T-2150

Product Information

• Name: 3,3',5,5'-Tetramethylbenzidine, dihydrochloride hydrate
• Synonyms: FT170921
• Brand: Biosynth
• Description: HRP and TMB – it takes two to tango! The enzyme horseradish peroxidase (HRP) has been isolated from the roots of the plant in commercially useful quantities since the nineteen-sixties. Today, HRP-coupled antibodies have become indispensable in immunoassays. The most popular chromogenic substrate for HRP is Tetramethylbenzidine (TMB), in particular in enzyme-linked Immunosorbent assays (ELISA). HRP converts TMB into a blue color that can be read spectrophotometrically at 650 nm. The HRP reaction can be stopped by adding sulfuric or phosphoric acid. This yields a yellow color that can be read at 450 nm.

Chemical properties

• Molecular weight: 313.27 g/mol
• Formula: C₁₆H₂₂Cl₂N₂(anhydrousbasis)