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The polymerase chain reaction (PCR) is a technique that amplifies specific DNA sequences. The process begins with the binding of primers to a target sequence in the DNA molecule. The primer sequences are chosen to be complementary to the desired sequence, and they can be either RNA or DNA. A mixture of deoxyribonucleotide triphosphates (dNTPs), a thermostable DNA polymerase, and buffer are added, and the mixture is heated to separate the two strands at their melting point. The temperature is then lowered, and the two primers anneal to their complement on both strands. This creates a double-stranded molecule called a "primer extension product." If there is no amplification of dna, then this process is repeated using new primers and dNTPs. When amplification does occur, each cycle doubles the number of copies of the original target sequence in the sample. In PCR, it takes about 30 cycles for half of all
Chemical properties
Technical inquiry about: 3D-TCA64911 1-Decene, dimer, hydrogenated
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