Human VD(Vitamin D) ELISA Kit

Ref. EK-ELK11264

Product Information

• Name: Human VD(Vitamin D) ELISA Kit
• Synonyms: VD; VD1; Lamisterol; Vitamin D; Vitamin D1; Lumisterol
• Brand: ELK Biotechnology
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VD in the samples is then determined by comparing the OD of the samples to the standard curve.