Mouse LEP(Leptin) ELISA Kit
Ref. EK-ELK1161
| 48T | 333.00 € | ||
| 96T | 472.00 € |
Product Information
- OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LEP in the samples is then determined by comparing the OD of the samples to the standard curve.
Chemical properties
Technical inquiry about: EK-ELK1161 Mouse LEP(Leptin) ELISA Kit
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