Mouse PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit
Ref. EK-ELK2231
| 48T | 402.00 € | ||
| 96T | 574.00 € |
Product Information
- PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2; Prostaglandin Endoperoxide Synthase 2
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Chemical properties
Technical inquiry about: EK-ELK2231 Mouse PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit
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