Human ET-1(Endothelin1) ELISA Kit
Ref. EK-ELK2444
| 48T | 402.00 € | ||
| 96T | 574.00 € |
Product Information
- ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Chemical properties
Technical inquiry about: EK-ELK2444 Human ET-1(Endothelin1) ELISA Kit
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