Human DNMT1(DNA Methyltransferase 1) ELISA Kit
Ref. EK-ELK3561
48T | 402.00 € | ||
96T | 574.00 € |
Product Information
- CXXC9; DNMT; MCMT; Cytosine-5 DNA Methyltransferase 1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DNMT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DNMT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DNMT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DNMT1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Chemical properties
Technical inquiry about: EK-ELK3561 Human DNMT1(DNA Methyltransferase 1) ELISA Kit
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