Rat FGF15(Fibroblast Growth Factor 15) ELISA Kit
Ref. EK-ELK7800
48T | 402.00 € | ||
96T | 575.00 € |
Product Information
- FGF-15; Fgf15; FGF15; FGF19; Fibroblast growth factor 15
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FGF15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGF15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FGF15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FGF15 in the samples is then determined by comparing the OD of the samples to the standard curve.
Chemical properties
Technical inquiry about: EK-ELK7800 Rat FGF15(Fibroblast Growth Factor 15) ELISA Kit
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