25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
Ref. EK-ELK9387
| 48T | 334.00 € | ||
| 96T | 474.00 € |
Product Information
- 25 Hydroxy Vitamin D;
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-OH-D protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
Chemical properties
Technical inquiry about: EK-ELK9387 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
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