EK-ELK9613 - mouse-papplasmin-antiplasmin-complex-elisa-kit

Alpha 2 antiplasmin antibody was raised in mouse using purified alpha-2 antiplasmin as the immunogen.

Alpha 2 antiplasmin antibody was raised in mouse using purified alpha-2 antiplasmin as the immunogen.

Alpha 2 antiplasmin antibody was raised in mouse using purified alpha-2 antiplasmin as the immunogen.

Alpha 2 antiplasmin antibody was raised in mouse using purified alpha-2 antiplasmin as the immunogen.

alpha 1 Antiplasmin antibody was raised in sheep using human alpha 1 Antiplasmin purified from plasma as the immunogen.

alpha 2 Antiplasmin antibody was raised in goat using human alpha 2 Antiplasmin purified from plasma as the immunogen.

alpha 2 Antiplasmin antibody was raised in sheep using human alpha 2 Antiplasmin purified from plasma as the immunogen.

Alpha 2 Antiplasmin protein is an acidic protein that exhibits natriuretic and growth factor-like properties. It has been shown to interact with the growth factor-1 receptor and promote cell proliferation in various cell types. Alpha 2 Antiplasmin protein can be found in human serum and can be detected using monoclonal antibodies. This protein is commonly used in Life Sciences research, particularly in studies involving electrode-based techniques and telomerase activity. Additionally, alpha 2 Antiplasmin protein has been implicated in regulating chemokine and insulin-like growth factor signaling pathways. It is a valuable tool for studying Native Proteins & Antigens and is widely used in the field of Proteins and Antigens research.

alpha 1 Antiplasmin Matched Pair antibody Set for ELISA

alpha 1 Antiplasmin antibody (HRP) was raised in sheep using human alpha 1 Antiplasmin purified from plasma as the immunogen.

alpha 2 Antiplasmin antibody (HRP) was raised in goat using human alpha 2 Antiplasmin purified from plasma as the immunogen.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PAP in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PAP in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAP in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PAP in the samples is then determined by comparing the OD of the samples to the standard curve.

Alpha 2 Antiplasmin serves as the principal circulating inhibitor of plasmin. This compound plays a crucial role in regulating fibrinolysis within blood vessels.

Alpha-2-antiplasmin (alpha2AP), a member of the serine protease inhibitor (SERPIN) superfamily, is the main inhibitor of the fibrinolytic enzyme plasmin as well as an inhibitor of trypsin, elastase, and C protein. It plays a crucial role in reducing plasmin production and activity and thus inhibiting fibrinolysis.alpha2AP is synthesized in the liver and secreted as a single-chain glycoprotein, containing 11-14% carbohydrate, with a methionine (Met) as its N-terminus (Met-alpha2AP). The N-terminus of alpha2AP is involved in the incorporation of alpha2AP into a clot and the C-terminus is involved in the initial interaction of alpha2AP with plasmin(ogen). Circulating alpha2AP undergoes both N-and C-terminal modifications, which alter its activity. Increased concentrations of a2AP are associated with a higher risk of cardiovascular diseases.