Ribonuclease H (RNase H) (5U/ul)
Ref. SR-38080
50units
101.00€
250units
287.00€
Product Information
Name:Ribonuclease H (RNase H) (5U/ul)
Brand:Sisco Research Laboratories
Description:RNase H is a 18.9 kDa recombinant endoribonuclease purified from an Escherichia coli strain, which over-expresses cloned RNase H gene (rnh). The enzyme hydrolyses specifically the phosphodiester bonds of RNA hybridized to DNA and produces 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA. Rnase H does not degrade single and double-stranded DNA or unhybridized RNA. It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. Treating cDNA with RNase H prior to PCR can improve sensitivity as RNA bonded to the cDNA template may prevent binding of the amplification primers in a PCR reaction. RNase H treatment is often necessary when amplifying longer, full-length cDNA targets. In addition, RNase H is useful for the removal of poly(A) tails on mRNAs after hybridization with oligo(dT) and also for the site-specific enzymatic cleavage of RNA.
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Chemical properties
Color/Form:Liquid, Colourless, Clear
Technical inquiry about: Ribonuclease H (RNase H) (5U/ul)
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