TRIzol-C Reagent
Ref. SR-41066
20ml
35.00€
100ml
89.00€
Product Information
Name:TRIzol-C Reagent
Brand:Sisco Research Laboratories
Description:TRIzol-C Reagent is our new and cost-effective ready-to-use formulation for isolation of pure total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement giving very high yields of pure RNA useful for downstream processes .
During sample homogenization or lysis, TRIzol-C Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Copurification of the DNA may be useful for normalizing RNA yields from sample to sample.
During sample homogenization or lysis, TRIzol-C Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Copurification of the DNA may be useful for normalizing RNA yields from sample to sample.
Notice:Our products are intended for lab use only. For any other use, please contact us.
Chemical properties
Color/Form:Liquid, Pale Yellow (Straw coloured)
Technical inquiry about: TRIzol-C Reagent
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