Product Information
- 5-Fluorouracil-6-carboxylic acid Mono(hydrate)
5-Fluoroorotic acid (monohydrate) (5-Fluorouracil-6-carboxylic acid Mono(hydrate); 5-FOA) is employed to detect the expression of the URA3 gene in yeast molecular genetic constructs. Yeast possessing an active URA3 gene (which encodes orotidine-5'-monophosphate decarboxylase) convert 5-FOA into 5-fluorodeoxyuridine, a substance toxic to cells. Yeast strains with mutations in the URA3 gene that confer resistance can grow in the presence of 5-FOA if uracil is added to the medium.
Chemical properties
Technical inquiry about: TM-T200989 5-Fluoroorotic acid monohydrate
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