(5S)-N-(5-Amino-1-carboxypentyl)iminodiacetic Acid

CAS: 113231-05-3

Ref. TR-A603250

Product Information

• Name: (5S)-N-(5-Amino-1-carboxypentyl)iminodiacetic Acid
• Synonyms: (2S)-6-amino-2-[bis(carboxymethyl)amino]hexanoic acid;hydrate
• Brand: TRC

Description

Applications A nitrilotriacetic acid derivative used as a metal chelating adsorbent for metal ion affinity chromatography. This method can be used for identification and rapid one-step purification of gene products expressed as fusion proteins with an oligo-histidine tag. The oligo-histidine tag serves as a high affinity binding sequence for the purification of fusion proteins via a metal chelating absorbent such as N-(5-Amino-1-carboxypentyl)iminodiacetic acid.Also soluble in DMSO:DMF 1:1 and DMSO:CH2Cl2 1:2
References Hochuli, E., et al.: J. Chromatography, 411, 177 (1987), Meredith, G.D., et al.: Bioconjugate Chem., 15, 969 (2004)

Chemical properties

• Molecular weight: 280.28
• Formula: C₁₀H₁₈N₂O₆·H₂O
• Color/Form: Neat
• InChI: InChI=1S/C10H18N2O6.H2O/c11-4-2-1-3-7(10(17)18)12(5-8(13)14)6-9(15)16;/h7H,1-6,11H2,(H,13,14)(H,15,16)(H,17,18);1H2/t7-;/m0./s1
• InChI key: InChIKey=SYFQYGMJENQVQT-ZETCQYMHSA-N
• SMILES: NCCCC[C@@H](C(=O)O)N(CC(=O)O)CC(=O)O