Ethidium Bromide

CAS: 1239-45-8

Ref. TR-E890700

Product Information

• Name: Ethidium Bromide
• Synonyms: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide, 2,7-Diamino-10-ethyl-9-phenylphenanthridinium Bromide, 2,7-Diamino-9-phenyl-10-ethylphenanthridinium Bromide, 2,7-Diamino-9-phenylphenanthridine Ethobromide, Dromilac, Homidium Bromide
• Brand: TRC
• Description: Applications Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma). Dyes and metabolites. Not a dangerous good if item is equal to or less than 1g/ml and there is less than 100g/ml in the packageReferences Waring, M.J., et al.: J. Mol. Biol., 13, 269 (1965), Probst, G.S., et al.: Environ. Mutagen., 3, 11 (1981),

Chemical properties

• Molecular weight: 394.31
• Formula: C₂₁H₂₀BrN₃
• Color/Form: Neat
• InChI: InChI=1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
• SMILES: CC[n+]1c(-c2ccccc2)c2cc(N)ccc2c2ccc(N)cc21.[Br-]