Ethidium-D5 Bromide

CAS: 1579939-34-6

Ref. TR-E890702

Product Information

• Name: Ethidium-D5 Bromide
• Synonyms: 3,8-Diamino-5-(ethyl-d5)-6-phenylphenanthridinium Bromide Hydrodromide Salt, 2,7-Diamino-10-(ethyl-d5)-9-phenylphenanthridinium Bromide Hydrodromide Salt, 2,7-Diamino-9-phenyl-10-(ethyl-d5)phenanthridinium Bromide Hydrodromide Salt, 2,7-Diamino-9-phenylphenanthridine-d5 Ethobromide Hydrodromide Salt, Dromilac-d5 Hydrodromide Salt, Homidium-d5 Bromide Hydrodromide Salt
• Brand: TRC
• Description: Applications Labelled Ethidium bromide (EtBr) (E890700). It is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma). Not a dangerous good if item is equal to or less than 1g/ml and there is less than 100g/ml in the packageReferences Waring, M.J., et al.: J. Mol. Biol., 13, 269 (1965), Probst, G.S., et al.: Environ. Mutagen., 3, 11 (1981),

Chemical properties

• Molecular weight: 399.34 + x(80.91)
• Formula: C₂₁H₁₅D₅BrN₃•x(HBr)
• Color/Form: Neat
• InChI: InChI=1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H/i1D3,2D2
• InChI key: InChIKey=ZMMJGEGLRURXTF-LUIAAVAXSA-N
• SMILES: Br.[2H]C([2H])([2H])C([2H])([2H])n1c2cc(=N)ccc-2c2ccc(N)cc2c1-c1ccccc1