Rhodamine-123

CAS: 62669-70-9

Ref. TR-R318585

Product Information

• Name: Rhodamine-123
• Synonyms: 3,6-Diamino-9-[2-(methoxycarbonyl)phenyl]xanthylium Chloride, 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic Acid Methyl Ester Monohydrochloride, R 22420, R 302, RH 123, 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester monohydrochloride, 3,6-Diamino-9-[2-(Methoxycarbonyl)Phenyl]Xanthenium Chloride, 6-amino-9-[2-(methoxycarbonyl)phenyl]-3H-xanthen-3-iminium chloride, Benzoic acid, 2-(6-amino-3-imino-3H-xanthen-9-yl)-, methyl ester, monohydrochloride, Rh 123, Rhodamine 123, Xanthylium, 3,6-diamino-9-(2-(methoxycarbonyl)phenyl)-, chloride, Xanthylium, 3,6-diamino-9-(2-(methoxycarbonyl)phenyl)-, chloride (1:1)

Description

Applications Rhodamine-123 is a fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Rhodamine 123 (R123), as a typical of P-glycoprotein substrate, was widely used to quantify P-glycoprotein (P-gp) functional efflux activity in vivo. Rhodamine-123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid).
References Yumoto, R., et al.: J. Pharmacol. Exp. Ther., 289, 149 (1999), Romiti, N., et al.: Toxicol. Appl. Pharmacol., 183, 83 (2002), Mizuno, N., et al.: Pharmacol. Rev., 559, 425 (2003), del Amo, E., et al.: Eur. J. Pharm. Sci., 36, 200 (2009),

• Brand: TRC

Chemical properties

• Molecular weight: 380.82
• Formula: C₂₁H₁₆N₂O₃·ClH
• Color/Form: Neat
• InChI: InChI=1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H
• SMILES: COC(=O)c1ccccc1-c1c2ccc(N)cc2[o+]c2cc(N)ccc12.[Cl-]