Substratos enzimáticos
Substratos enzimáticos são moléculas sobre as quais as enzimas atuam para catalisar reações químicas. O substrato liga-se ao sítio ativo da enzima, onde passa por uma transformação específica para formar o produto da reação. Os substratos enzimáticos são ferramentas essenciais na pesquisa para estudar a cinética enzimática, especificidade e mecanismo de ação. Eles também são usados em ensaios diagnósticos e estudos bioquímicos. Na CymitQuimica, oferecemos uma seleção diversificada de substratos enzimáticos de alta qualidade para apoiar suas pesquisas em enzimologia e bioquímica.
Subcategorias de "Substratos enzimáticos"
Foram encontrados 1588 produtos de "Substratos enzimáticos"
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4-Methylumbelliferyl 6-O-(N-acetylneuraminyl)-b-D-glucopyranoside
4-Methylumbelliferyl 6-O-(N-acetylneuraminyl)-b-D-glucopyranoside is a fluorescent substrate for the enzyme β-D-galactosidase. It is used in the detection of β-D-galactosidase activity in the diagnosis of various diseases, including celiac disease and AIDS. 4-Methylumbelliferyl 6-O-(N-acetylneuraminyl)-b-D-glucopyranoside can also be used as a chromogenic or fluorogenic substrate for chemiluminescent detection of β-D-galactosidase activity. The conjugate with horseradish peroxidase can be used to detect hydrogen peroxide in water samples, such as drinking water, wastewater, and surface waters. 4MUG has been shown to have high purity and is CAS No. 78761–35–4.Pureza:Min. 95%Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride
CAS:Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.Fórmula:C14H16ClN3O4Pureza:Min. 95%Cor e Forma:PowderPeso molecular:325.75 g/mol4-Methylumbelliferyl-α-D-mannopyranoside
CAS:4-Methylumbelliferyl alpha-D-mannopyranoside (4-MU-a-D-Man) is a fluorogenic substrate used in activity assays for alpha-mannosidase. When it is hydrolyzed by alpha-mannosidase, it produces a fluorescent compound, 4-methylumbelliferone (4-MU). 4-MU-a-D-Man is often used in medical research to monitor the activity of alpha-mannosidase, which is important in lysosomal storage diseases. In addition, 4-Methylumbelliferyl alpha-D-mannopyranoside has been used to study the the activity of concanavalin A, a lectin that specifically binds to alpha-D-mannopyranosyl and alpha-D-glucopyranosyl residues on various glycoproteins. The use of this fluorogenic enzyme substrate permits study the degree of alpha-D-mannosidic linkage cleavage by the enzyme.Fórmula:C16H18O8Pureza:Min. 98 Area-%Peso molecular:338.32 g/molRef: 3D-M-5730
-Unit-ggA consultar1gA consultar5gA consultar10gA consultar500mgA consultar2500mgA consultar4-Methylumbelliferyl a-D-glucosaminide
CAS:4-Methylumbelliferyl alpha-D-glucosaminide is a fluorogenic substrate for alpha-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-D-glucosaminideas a substrate for measuring the alpha-N-acetylglucosaminidase activity is used for Sanfilippo syndrome B and Mucopolysaccharidosis IIIB (MPS IIIB) diagnosis.Fórmula:C16H19NO7Pureza:Min. 90 Area-%Cor e Forma:White PowderPeso molecular:337.32 g/molAldol® 484 beta-D-galactopyranoside, Biosynth Patent: EP 2427431 and US 8940909
CAS:Aldol® 484 beta-D-galactopyranoside is a chromogenic enzyme substrate for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color. Aldol® 484 beta-D-galacto-pyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar. Aldol® 484 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.Fórmula:C26H24ClFN2O7Pureza:Min. 95%Cor e Forma:PowderPeso molecular:530.93 g/mol2-Chloro-4-nitrophenyl β-D-glucopyranoside
CAS:2-Chloro-4-nitrophenyl β-D-glucopyranoside is a chromogenic substrate for beta-glucosidases. In biochemical enzyme assays and in vitro diagnostics analysis, the substrate is used to monitor the glycosyltransferase activity. When 2-chloro-4-nitrophenyl β-D-glucopyranoside is cleaved by beta-glucosidase, 2-chloro-4-nitrophenol is released and quantified.Fórmula:C12H14ClNO8Pureza:Min. 95%Cor e Forma:White PowderPeso molecular:335.69 g/mol4-Methylumbelliferyl-beta-D-xylopyranoside
CAS:Fluorogenic substrate for β-D-xylosidase. Identification within the family of Enterobacteriaceae.Fórmula:C15H16O7Pureza:Min. 99.0 Area-%Peso molecular:308.29 g/molL-Alanine-7-amido-4-methylcoumarin hydrochloride
CAS:L-Alanine-7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for alanine aminopeptidase.Fórmula:C13H14N2O3·HClPureza:Min. 90%Cor e Forma:PowderPeso molecular:282.72 g/mol4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(b-D-galactopyranosyl)-b-D-glucopyranoside
CAS:Chromogenic substrate for fucosyltransferasesFórmula:C20H28N2O13Pureza:Min. 98 Area-%Cor e Forma:White PowderPeso molecular:504.44 g/mol4-Nitrophenyl 2-O-trans-feruloyl-a-L-arabinofuranoside
CAS:4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is a chromogenic substrate for alpha-L-arabinofuranosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is used in different applications such as the Plant biomass analysis, bacterial arabinan degradation assays
Fórmula:C21H21NO10Pureza:Min. 95%Cor e Forma:PowderPeso molecular:447.39 g/mol4-Methylumbelliferyl 2-deoxy-2-sulfamino-a-D-glucopyranoside sodium salt - Moscerdam™ biochemical purity
CAS:4-Methylumbelliferyl 2-deoxy-2-sulfamino-a-D-glucopyranoside sodium salt (M3A or MU-GlcNS) is a fluorogenic substrate of heparin sulfamidase, used in the diagnosis of Mucopolysaccharidosis III type A (MPS III A). MPS III A, also known as Sanfilippo syndrome type A, is caused by the deficiency of heparan N-sulfatase. Heparin sulfamidase in the assay desulfates MU-GlcNS to 4-methylumbelliferyl 2-amino-2-deoxy-α-D-glucopyranoside (MU-GlcNH2). To release fluorometrically measurable 4-methylumbelliferone (MU) and by-product glucosamine, MU-GlcNH2 is further hydrolysed by an enzyme such as yeast α-glucosidase during a second incubation.Fórmula:C16H18NO10SNaPureza:Min. 95 Area-%Cor e Forma:White PowderPeso molecular:439.37 g/molAc-DEVD-6-Amino-D-Luciferin
Caspases are a family of protease enzymes playing essential roles in programmed cell death and inflammation. The acronym caspase stands for Cysteine Aspartic Acid Specific Protease due to their specific cleavage of an aspartic acid residue, by mean of a cysteine in the catalytic site of the enzyme._x000D_About 11 or 12 different caspases are known in humans and 10 in mice, participating in numerous different cellular functions. Caspase deficiency, for example, has been identified as a cause of tumor development, while over-activation of some caspases such as caspase-3 can lead to excessive apoptosis that can be observed in several neurodegenerative diseases. Caspases cleave proteins at specific peptide recognition sequences. Ac-DEVD-6-Amino-D-Luciferin (Ac-Asp-Glu-Val-Asp-6-Amino-D-Luciferin, Cat.No_x000D_L-8282) is a synthetic chemiluminescent peptide for the determination of caspase-3 and caspase-7activity.
Fórmula:C31H37N7O13S2Peso molecular:779.79 g/molRef: 3D-L-8282
-Unit-ggA consultar10mgA consultar25mgA consultar50mgA consultar100mgA consultar250mgA consultar4-Nitrophenyl-beta-D-galactopyranoside
CAS:Chromogenic β-galactosidase substrate yielding a yellow solution upon cleavage. Can be used for the detection of coliforms due to their β-galactosidase activity.Fórmula:C12H15NO8Pureza:Min. 98.0 Area-%Peso molecular:301.26 g/molCelluchrome
Celluchrome (short name for remazol brilliant blue carboxymethyl cellulose or RBB-CMC) is a chromogenic substrate for cellulases. With a higher sensitivity, RBB-CMC allows for the detection of the enzyme activity even when small amounts are present.Pureza:Min. 95%Cor e Forma:PowderIsopropyl-β-D-thiogalactopyranoside, <5ppm dioxane, plant origin (ex peach gum)
CAS:A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.Fórmula:C9H18O5SPureza:Min. 99.0 Area-%Peso molecular:238.30 g/molRef: 3D-I-8070
-Unit-ggA consultar1kgA consultar50gA consultar100gA consultar250gA consultar500gA consultarCalcium 2-naphthylphosphate
CAS:Calcium 2-naphthylphosphate, which is the calcim salt of 2-naphthylphosphate, is a chromogenic substrate to measure alkaline phosphatase activity. After enzymatic cleavage, the product yielded (2-naphthol) generates a red-colored complex upon reaction with a diazonium salt like Fast Blue RR or Fast Garnet GBC.
Pureza:(%) Min. 95%Cor e Forma:PowderPeso molecular:282.24 g/molGlycine 7-amido-4-methylcoumarin hydrobromide
CAS:Glycine 7-amido-4-methylcoumarin hydrobromide is a chromogenic substrate that can be used for the detection of alkaline phosphatase. It reacts with the enzyme to produce a blue color, which can be measured spectrophotometrically. The product is also used as a ligand in cell culture media and as a diagnostic agent in environmental testing. It has been shown to be an excellent substrate for chemiluminescence reactions with luminol and luciferin, and bioluminescence reactions with luciferase. Glycine 7-amido-4-methylcoumarin hydrobromide is available from several suppliers at different prices, but we are able to offer it at a competitive price due to our high quality standards. The following product description was generated for 6-Fluoro-3-indoxyl-beta-D-galactopyranoside: RifFórmula:C12H13BrN2O3Pureza:Min. 98.0 Area-%Peso molecular:313.16 g/mol4-Methylumbelliferyl phosphate, disodium salt trihydrate
CAS:Sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases.Fórmula:C10H13Na2O9PPeso molecular:354.16 g/mol4-Methylumbelliferyl dodecanoate
CAS:4-Methylumbelliferyl dodecanoate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl dodecanoateas a substrate for measuring the lipase activity is used for detecting pancreatic insufficiency in diagnosis assays as well as the screening of lipase deficiency.Fórmula:C22H30O4Pureza:Min. 95%Cor e Forma:PowderPeso molecular:358.47 g/molResorufin N-acetyl-b-D-glucosamine
CAS:Resorufin N-acetyl-b-D-glucosamine is fluorogenic substrate for N-acetyl-beta-D-glucosaminidase (NAG).
Fórmula:C20H20N2O8Pureza:Min. 98 Area-%Cor e Forma:Orange PowderPeso molecular:416.38 g/mol
