CAS 104992-55-4

N-Benzoyl-5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]adenosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite

Description:

N-Benzoyl-5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]adenosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite, with CAS number 104992-55-4, is a phosphoramidite derivative of adenosine, commonly used in the synthesis of oligonucleotides. This compound features several protective groups, including a benzoyl group and a dimethoxytrityl group, which help to stabilize the molecule during the synthesis process. The tert-butyl dimethylsilyl group provides additional protection for the 2'-hydroxyl group, enhancing the compound's stability and reactivity. The presence of the cyanoethyl group is crucial for the phosphoramidite's role in the phosphoramidite chemistry, facilitating the coupling reaction during oligonucleotide synthesis. This compound is typically utilized in automated synthesizers for the production of DNA and RNA sequences, making it an essential reagent in molecular biology and genetic research. Its complex structure allows for selective reactions, ensuring high fidelity in the synthesis of nucleic acid sequences.

Formula: C₅₃H₆₆N₇O₈PSi

Synonyms:

• 2'-TBDMS-rA(N-Bz) Phosphoramidite
• 5'-O-DMT-2'-TBDMS-rA(N-Bz)-3'-CEDPA
• Bz-rA Phosphoramidite
• N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(t-butyl-dimethylsilyl)-adenosine-3'-cyanoethyl Phosphoramidite

Dmt-2'o-tbdms-ra(bz) phosphoramidite

CAS: 104992-55-4

Formula: C₅₃H₆₆N₇O₈PSi

Purity: 96%

Color and Shape: Solid

Molecular weight: 988.1925

Ref: IN-DA0038CQ

DMT-2'O-TBDMS-rA(bz) Phosphoramidite

CAS: 104992-55-4

DMT-2'O-TBDMS-rA(bz) Phosphoramidite

Purity: 97%

Color and Shape: Solid

Molecular weight: 987.20g/mol

Ref: 54-BIDI2000

N6-Benzoyl-2''-O-tert-butyldimethylsilyl-5''-O-DMT-adenosine 3''-CE phosphoramidite

CAS: 104992-55-4

Formula: C₅₃H₆₆N₇O₈PSi

Purity: ≥ 98.0%

Color and Shape: White to off-white powder

Molecular weight: 988.22

Ref: 7W-GN4743

Bz-rA Phosphoramidite

CAS: 104992-55-4

Bz-rA Phosphoramidite is used for ribonucleotides modification[1].

Formula: C₅₃H₆₆N₇O₈PSi

Color and Shape: Solid

Molecular weight: 988.19

Ref: TM-T37107

N6-Benzoyl-2'-O-tert-butyldimethylsilyl-5'-O-DMT-adenosine 3'-CE phosphoramidite

CAS: 104992-55-4

Nucleoside phosphoramidites are used in the synthesis of oligonucleotides. Using a chemical approach rather than an enzymatic one allows quick and inexpensive access to custom-made oligonucleotides of the desired sequence. Contrarily to the enzymatic synthesis, the chemically synthesised oligonucleotide sequence is typically constructed in a 3’ to 5’ direction. N6-Benzoyl-2'-O-tert-butyldimethylsilyl-5'-O-DMT-adenosine 3'-CE phosphoramidite, also called DMT-2’O-TBDMS-rA(bz) amidite, is used in the synthesis of cyclic di-AMP, a second messenger used in signal transduction in bacteria.

Formula: C₅₃H₆₆N₇O₈PSi

Purity: Min. 98 Area-%

Color and Shape: White Powder

Molecular weight: 988.22 g/mol

Ref: 3D-PB08707

Bz-rA Phosphoramidite

CAS: 104992-55-4

Purity: 96%

Molecular weight: 987.45

Ref: 10-F556390